Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Daniel Klaue"'
Autor:
Ulrich F. Keyser, Christoph Herold, Stefano Pagliara, Alexander Mietke, Stefan Golfier, Jörg Mansfeld, Angela Jacobi, Elisabeth Fischer-Friedrich, Nicole Töpfner, Andrew Ekpenyong, Philipp Rosendahl, Manja Wobus, Daniel Klaue, Oliver Otto, Jochen Guck, Salvatore Girardo
Publikováno v:
Nature Methods
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distingui
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c37ae62908e32ae90300a3d1186c93ea
https://doi.org/10.1038/nmeth.3281
https://doi.org/10.1038/nmeth.3281
Publikováno v:
Biophysical Journal. 98(7):1267-1276
Twisting a DNA molecule held under constant tension is accompanied by a transition from a linear to a plectonemic DNA configuration, in which part of the applied twist is absorbed in a superhelical structure. Recent experiments revealed the occurrenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f7b76474fffa223ba2a156020a2b60c
Autor:
Christoph Herold, Stefan Golfier, Daniel Klaue, Oliver Otto, Jochen Guck, Angela Jacobi, Nicole Töpfner, Elisabeth Fischer-Friedrich, Alexander Mietke, Philipp Rosendahl
Publikováno v:
ResearcherID
Biophysical Journal
Biophysical Journal
Changes in cell function are often accompanied by alterations of the intrinsic cell structure particularly the cytoskeleton. This leads to distinct mechanical changes. For example, cells become softer during malignant transformation and stiffer durin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddff359756cae856956338e2994b2d2f
Publikováno v:
Proceedings of the National Academy of Sciences. 110
Dna2 is a nuclease-helicase involved in several key pathways of eukaryotic DNA metabolism. The potent nuclease activity of Saccharomyces cerevisiae Dna2 was reported to be required for all its in vivo functions tested to date. In contrast, its helica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c3833022a1469b22229fc4cab225784
https://doi.org/10.1073/pnas.1300390110
https://doi.org/10.1073/pnas.1300390110
Autor:
Daniel Klaue, Felix E. Kemmerich, Ralf Seidel, Alicja Kozikowska, Daniela Kobbe, Holger Puchta
Publikováno v:
Nature Communications
RecQ helicases have essential roles in maintaining genome stability during replication and in controlling double-strand break repair by homologous recombination. Little is known about how the different RecQ helicases found in higher eukaryotes achiev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d10bf5609b056df4847a87e81b525d2d
https://pubmed.ncbi.nlm.nih.gov/23771268
https://pubmed.ncbi.nlm.nih.gov/23771268
Autor:
Ralf Seidel, Daniel Klaue
Publikováno v:
Physical Review Letters. 108
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b628112737964582ccc73c9baf5ed747
https://doi.org/10.1103/physrevlett.108.209902
https://doi.org/10.1103/physrevlett.108.209902
Autor:
Daniel Klaue, Ralf Seidel
Publikováno v:
Physical Review Letters. 102
We used magnetic tweezers to measure the torsional stiffness of single micrometer-sized superparamagnetic spheres as a function of the applied magnetic field. By investigating the axial fluctuations of DNA-bound microspheres, we found that considerab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b71173aa0e9266cb2abb46275be3dc4
https://doi.org/10.1103/physrevlett.102.028302
https://doi.org/10.1103/physrevlett.102.028302
Autor:
Peter Daldrop, Daniel Klaue, Ralf Seidel, Hergen Brutzer, Oliver Otto, Alexander Huhle, Sihwa Joo, Ulrich F. Keyser
Publikováno v:
Nature communications
Optical and magnetic tweezers are widely employed to probe the mechanics and activity of individual biomolecular complexes. They rely on micrometre-sized particles to detect molecular conformational changes from the particle position. Real-time parti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f93f5cd493cca7e396279616d062563
http://europepmc.org/articles/PMC4338538
http://europepmc.org/articles/PMC4338538
Publikováno v:
Diffusion fundamentals 20 (2013) 72, S. 1
Friction within biomolecules has recently gained increasing interest. Here we present a method that allows to study the friction that occurs during fast, few nm-sized refolding processes of nucleic acids. Branch migration of a homologous Holliday jun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e86e6465cfc66edcec8454ff35f64d18
https://doi.org/10.1016/j.bpj.2012.11.1475
https://doi.org/10.1016/j.bpj.2012.11.1475
Autor:
Ralf Seidel, Sylvia Clausing, Friedrich W. Schwarz, Nicholas Luzzietti, Wolfgang Staroske, Daniel Klaue, Hergen Brutzer
Publikováno v:
Nucleic Acids Research
Investigations of enzymes involved in DNA metabolism have strongly benefited from the establishment of single molecule techniques. These experiments frequently require elaborate DNA substrates, which carry chemical labels or nucleic acid tertiary str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b269941b761fa25c2b57cd45385d602
https://doi.org/10.1093/nar/gkq1004
https://doi.org/10.1093/nar/gkq1004