Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Daniel Kage"'
Autor:
Daniel Kage, Andrej Eirich, Kerstin Heinrich, Jenny Kirsch, Jan Popien, Alexander Wolf, Konrad v. Volkmann, Hyun-Dong Chang, Toralf Kaiser
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-11 (2024)
Abstract Flow cytometry is a key technology for the analysis and sorting of cells or particles at high throughput. Conventional and current flow cytometry is primarily based on fluorescent stains to detect the cells of interest. However, such stains
Externí odkaz:
https://doaj.org/article/0c9f71e28a344bf9a11b6857d98b2958
Autor:
Jenny Kirsch, Kerstin Heinrich, Daniel Kage, Johannes Glaab, Benjamin Grothe, Konrad v. Volkmann, Toralf Kaiser
Publikováno v:
Engineering in Life Sciences, Vol 22, Iss 8, Pp 550-553 (2022)
Abstract Aseptic cell sorting is challenging, especially when a flow‐cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fung
Externí odkaz:
https://doaj.org/article/802b90c8b4c04f1a90e0dbd39ed726cf
Autor:
Daniel Kage, Kerstin Heinrich, Konrad v. Volkmann, Jenny Kirsch, Kristen Feher, Claudia Giesecke-Thiel, Toralf Kaiser
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis. The combination of hardware modification and data analysis allows clas
Externí odkaz:
https://doaj.org/article/233a6ba2a990438f8bf02511361ce7c9
Autor:
Claudia Giesecke-Thiel, Kristen Feher, Kerstin Heinrich, Konrad von Volkmann, Toralf Kaiser, Daniel Kage, Jenny Kirsch
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Communications Biology
Communications Biology
Flow cytometers are robust and ubiquitous tools of biomedical research, as they enable high-throughput fluorescence-based multi-parametric analysis and sorting of single cells. However, analysis is often constrained by the availability of detection r
Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads
Autor:
Victor Krivenkov, Katrin Hoffmann, Galina Nifontova, Ute Resch-Genger, Alyona Sukhanova, Igor Nabiev, Daniel Kage
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports
Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-019-56938-2⟩
Scientific Reports
Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-019-56938-2⟩
International audience; Semiconductor quantum dots (QDs) embedded into polymer microbeads are known to be very attractive emitters for spectral multiplexing and colour encoding. Their luminescence lifetimes or decay kinetics have been, however, rarel
Autor:
Martin Presselt, Todd J. Martínez, Julia Preiss, Ute Resch-Genger, Katrin Hoffmann, Daniel Kage
Publikováno v:
The Journal of Physical Chemistry A. 122:9813-9820
The fluorescence lifetime is a key property of fluorophores that can be utilized for microenvironment probing, analyte sensing, and multiplexing as well as barcoding applications. For the rational design of lifetime probes and barcodes, theoretical m
Publikováno v:
The Journal of Physical Chemistry C. 122:12782-12791
Dye-stained micrometer-sized polymer beads are important tools in the life sciences with applications in biomedical, biochemical, and clinical research. Here, bead-based assays are increasingly used, for example, in DNA sequencing and the detection o
Autor:
Charlotte F. Gers-Panther, Walter Frank, Katrin Hoffmann, Ute Resch-Genger, Nithiya Nirmalananthan, Thomas Müller, Daniel Kage, Thomas Behnke
Publikováno v:
The Journal of Physical Chemistry C. 122:11119-11127
Aggregation-induced emission (AIE) has been meanwhile observed for many dye classes and particularly for fluorophores containing propeller-like groups. Herein, we report on the AIE characteristics of a series of four hydrophobic pyrrolidinylvinylquin
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
Scientific Reports
Time-resolved flow cytometry represents an alternative to commonly applied spectral or intensity multiplexing in bioanalytics. At present, the vast majority of the reports on this topic focuses on phase-domain techniques and specific applications. In
Publikováno v:
The Journal of Physical Chemistry C. 123:16494-16494