Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Daniel J. Wiegand"'
Autor:
Howon Lee, Daniel J. Wiegand, Kettner Griswold, Sukanya Punthambaker, Honggu Chun, Richie E. Kohman, George M. Church
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Writing data in DNA is still a bottleneck due to the reliance on chemical synthesis methods. Here the authors report multiplexed enzymatic DNA synthesis using maskless photolithography.
Externí odkaz:
https://doaj.org/article/f0694cb516a3421f8daca6541c619c5f
Autor:
Daniel J. Wiegand, Honggu Chun, Sukanya Punthambaker, George M. Church, Howon Lee, Kettner Griswold, Richie E. Kohman
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing
Autor:
Erkin Kuru, Karen Noguera, Rosa-Maria Määttälä, Kamesh Narasimhan, George M. Church, Devon A. Stork, Jonathan Rittichier, Daniel J. Wiegand
Publikováno v:
ACS chemical biology. 15(7)
We report a tunable chemical genetics approach for enhancing genetic code expansion in different wild-type bacterial strains that employ apidaecin-like, antimicrobial peptides observed to temporarily sequester and thereby inhibit Release Factor 1 (RF
Autor:
Sukanya Punthambaker, Stefan Roever, James Pollard, Timothy Craig, Adam Qwan, Preethi Sarvabhowman, Shaw Sun, Andrew Trans, Jeff Nivala, Sara Kelley, Eileen Thai, Mirko Palla, P. Benjamin Stranges, Daniel J. Wiegand, Ilya Lederman, Frederic Vigneault, Aruna Ayer, Colin Mcgaw, Helen Franklin, Charles Schwab, George M. Church, Evan Amato, Dhruti Dalal, Cleoma Arnold, Carl W. Fuller, Dmitriy Gremyachinskiy, Meng Taing
DNA polymerases have revolutionized the biotechnology field due to their ability to precisely replicate stored genetic information. Screening variants of these enzymes for unique properties gives the opportunity to identify polymerases with novel fea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7aadfe057b29ffe39fde4476cc3c707
https://doi.org/10.1101/2020.03.15.993071
https://doi.org/10.1101/2020.03.15.993071
Publikováno v:
ACS Synthetic Biology. 7:2475-2479
The fast growing bacterium Vibrio natriegens is an emerging microbial host for biotechnology. Harnessing its productive cellular components may offer a compelling platform for rapid protein production and prototyping of metabolic pathways or genetic
Publikováno v:
Journal of Visualized Experiments.
The marine bacterium Vibrio natriegens has garnered considerable attention as an emerging microbial host for biotechnology due to its fast growth rate. A general protocol is described for the preparation of V. natriegens crude cell extracts using com
Publikováno v:
Journal of Visualized Experiments.
The fast growing bacteriumVibrio natriegensis an emerging microbial host for biotechnology. Harnessing its productive cellular components may offer a compelling platform for rapid protein production and prototyping of metabolic pathways or genetic ci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::895068df5536a2f4d56d24e7fb2a8e49
Autor:
Jonathan L. Braff, John Aach, Norbert Perrimon, Dmitry Ter-Ovanesyan, Benjamin E. Housden, Samira Kiani, Daniel J. Wiegand, Christopher D. Guzman, Shuailiang Lin, Jonathan Scheiman, James J. Collins, Eswar Prasad Ramachandran Iyer, Noah Davidsohn, Alejandro Chavez, Ron Weiss, George M. Church, Benjamin W. Pruitt, Suhani Vora, Marcelle Tuttle
Publikováno v:
Nature methods
The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational de
Autor:
John Aach, Marcelle Tuttle, Benjamin W. Pruitt, Christopher D. Guzman, Daniel J. Wiegand, James J. Collins, Jonathan L. Braff, Suhani Vora, Samira Kiani, George M. Church, Noah Davidsohn, Jonathan Scheiman, Eswar Prasad Ramachandran Iyer, Alejandro Chavez, Ron Weiss, Dmitry Ter-Ovanesyan
The RNA-guided bacterial nuclease Cas9 can be reengineered as a programmable transcription factor by a series of changes to the Cas9 protein in addition to the fusion of a transcriptional activation domain (AD). However, the modest levels of gene act
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b342c6ffa1672d722f7eba65570c1048