Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Daniel Ivanusic"'
Publikováno v:
iScience, Vol 27, Iss 3, Pp 109123- (2024)
Summary: Conventional neutralizing enzyme-linked immunosorbent assay (ELISA) systems for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mimic the protein-protein interaction between angiotensin-converting enzyme 2 (ACE2) and the recepto
Externí odkaz:
https://doaj.org/article/2648e12d286b4e5ca3254fd1a5c8a3c1
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
Abstract Human immunodeficiency virus type 1 (HIV-1) persists lifelong in infected individuals and has evolved unique strategies in order to evade the immune system. One of these strategies is the direct cell-to-cell spread of HIV-1. The formation of
Externí odkaz:
https://doaj.org/article/cf4f484a765a4ecbb333522ff6eab171
Autor:
Daniel Ivanusic, Kazimierz Madela, Heidi Burghard, Gudrun Holland, Michael Laue, Norbert Bannert
Publikováno v:
BioTechniques, Vol 70, Iss 1, Pp 21-28 (2021)
A novel tool for the presentation of peptides and small proteins on the surface of human cells has been developed. Our tANCHOR system utilizes tetraspanin anchors containing heterologous amino acid sequences inserted instead of the large extracellula
Externí odkaz:
https://doaj.org/article/2131ad4919a7427aa599963ca23cd72e
Autor:
Christopher Janich, Daniel Ivanusic, Julia Giselbrecht, Elena Janich, Shashank Reddy Pinnapireddy, Gerd Hause, Udo Bakowsky, Andreas Langner, Christian Wölk
Publikováno v:
Pharmaceutics, Vol 12, Iss 9, p 805 (2020)
One major disadvantage of nucleic acid delivery systems is the low transfection or transduction efficiency of large-sized plasmids into cells. In this communication, we demonstrate the efficient transfection of a 15.5 kb green fluorescent protein (GF
Externí odkaz:
https://doaj.org/article/844a22204aa440ac91ce627baa42a9a4
Publikováno v:
PLoS ONE, Vol 13, Iss 7, p e0200570 (2018)
Immunosuppression by retroviruses including the human immunodeficiency virus-1 (HIV-1) is well known, however the mechanisms how retroviruses induce this immunosuppression is not fully investigated. It was shown that non-infectious retroviral particl
Externí odkaz:
https://doaj.org/article/4f3d854d3eff4829815881702055fa00
Publikováno v:
BioTechniques, Vol 59, Iss 2, Pp 63-73 (2015)
Yeast-based methods are still the workhorse for the detection of protein-protein interactions (PPIs) in vivo. Yeast two-hybrid (Y2H) systems, however, are limited to screening for a specific group of molecules that interact in a particular cell compa
Externí odkaz:
https://doaj.org/article/6727c034d1ef46eea8023f028bdd5163
Publikováno v:
BioTechniques, Vol 57, Iss 4, Pp 188-198 (2014)
Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein—protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between m
Externí odkaz:
https://doaj.org/article/c6e90e8a9b684bb39b015d83ba3f85fc
Publikováno v:
PLoS ONE, Vol 10, Iss 4, p e0122059 (2015)
Xenotransplantation has been proposed as a solution to the shortage of suitable human donors for transplantation and pigs are currently favoured as donor animals. However, xenotransplantation may be associated with the transmission of zoonotic microo
Externí odkaz:
https://doaj.org/article/175673f2a38a432684ef500c70737e6e
Autor:
Uwe Fiebig, Angela Holzer, Daniel Ivanusic, Elena Plotzki, Hartmut Hengel, Frank Neipel, Joachim Denner
Publikováno v:
Viruses, Vol 9, Iss 11, p 317 (2017)
Porcine cytomegalovirus (PCMV) infection is widely prevalent among pigs, and PCMV is one of the viruses which may be transmitted during xenotransplantation using pig cells, tissues, or organs. While human cytomegalovirus (HCMV) is a major risk factor
Externí odkaz:
https://doaj.org/article/c98d101915a54236a7e0be084ab3a26a
Publikováno v:
microPublication biology. 2022
Time-lapse microscopy for monitoring of fluorescently tagged proteins provides an undoubtedly important tool to understand transport, localization, and fate of proteins in living cells. Serial images are taken at consecutive time points to follow the