Zobrazeno 1 - 10
of 89
pro vyhledávání: '"Daniel I.C"'
Autor:
Markely, Lam Raga A., Ong, Boon Tee, Hoi, Kong Meng, Teo, Gavin, Lu, Michelle Y., Wang, Daniel I.C.
Publikováno v:
In Analytical Biochemistry 2010 407(1):128-133
Publikováno v:
In Biochemical Engineering Journal 2009 48(1):13-21
Publikováno v:
In Metabolic Engineering 2007 9(3):304-316
Mammalian cell culture is typically operated at the physiological temperature of 37°C. Low temperature cell culture at 30-33°C, in particular for CHO cells, increased the specific productivity of many recombinant proteins amongst many other benefit
Externí odkaz:
http://hdl.handle.net/1721.1/30382
When grown under hypothermic conditions, Chinese Hamster Ovary (CHO) cells become growth arrested in the G₀/G₁ phase of the cell cycle and also often exhibit increased recombinant protein production. In this study, we have validated this hypothes
Externí odkaz:
http://hdl.handle.net/1721.1/30379
In the field of biologics production, productivity and stability of the transfected gene of interest are two very important attributes that dictate if a production process is viable. To further understand and improve these two traits, we would need t
Externí odkaz:
http://hdl.handle.net/1721.1/7504
We have developed a system to hunt and reuse special gene integration sites that allow for high and stable gene expression. A vector, named pRGFP8, was constructed. The plasmid pRGFP8 contains a reporter gene, gfp2 and two extraneous DNA fragments. T
Externí odkaz:
http://hdl.handle.net/1721.1/7491
Most glyco-engineering approaches used to improve quality of recombinant glycoproteins involve the manipulation of glycosyltransferase and/or glycosidase expression. We investigated whether the over expression of nucleotide sugar transporters, partic
Externí odkaz:
http://hdl.handle.net/1721.1/7487
We present a statistical mechanical approach for quantifying thermodynamic properties of proteins in mixed solvents. This approach, based on molecular dynamics simulations which incorporate all atom models and the theory of preferential binding, allo
Externí odkaz:
http://hdl.handle.net/1721.1/3945
This study addresses the fundamentals of an important degradation pathway of storing protein pharmaceuticals in an aqueous formulation, oxidation of methionine residues by peroxides. First, a mechanism by which methionine residues are oxidized is ide
Externí odkaz:
http://hdl.handle.net/1721.1/3940