Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Daniel E. Ryan"'
Autor:
Daniel E. Ryan, Tamar Diamant-Levi, Israel Steinfeld, David Taussig, Savita Visal-Shah, Suhani Thakker, Benjamin D. Lunstad, Robert J. Kaiser, Ryan McCaffrey, Michael Ortiz, Justin Townsend, William R. W. Welch, Mandeep Singh, Bo Curry, Douglas J. Dellinger, Laurakay Bruhn
Publikováno v:
Biochemistry.
CRISPR gene editing and control systems continue to emerge and inspire novel research and clinical applications. Advances in CRISPR performance such as optimizing the duration of activity in cells, tissues, and organisms, as well as limiting off-targ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9a0e834fb976f7307540c96931b0e90
https://doi.org/10.1021/acs.biochem.1c00768
https://doi.org/10.1021/acs.biochem.1c00768
Autor:
Daniel E. Ryan, Daniel P. Dever, M. Kyle Cromer, Bo Curry, Sriram Vaidyanathan, Israel Steinfeld, Milan Kaushik, Joab Camarena, Matthew H. Porteus, Laurakay Bruhn, Anne Bergstrom Lucas, Sarah R. Hay, Rasmus O. Bak, Renata M. Martin, Ayal Hendel
Publikováno v:
Molecular Therapy. 26:2431-2442
Genome-editing technologies are currently being translated to the clinic. However, cellular effects of the editing machinery have yet to be fully elucidated. Here, we performed global microarray-based gene expression measurements on human CD34(+) hem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01e3a4eb32953246de646c59af5f3173
https://doi.org/10.1016/j.ymthe.2018.06.002
https://doi.org/10.1016/j.ymthe.2018.06.002
Autor:
Gregory S Richmond, Htet Khine, Tina T Zhou, Daniel E Ryan, Tony Brand, Mary T McBride, Kevin Killeen
Publikováno v:
PLoS ONE, Vol 6, Iss 4, p e18967 (2011)
Multiplexed detection assays that analyze a modest number of nucleic acid targets over large sample sets are emerging as the preferred testing approach in such applications as routine pathogen typing, outbreak monitoring, and diagnostics. However, ve
Externí odkaz:
https://doaj.org/article/d874aa978fc34d50be98f8218145cf98
Autor:
Benjamin D. Lunstad, Israel Steinfeld, Kenji D Okochi, Laurakay Bruhn, Subhadeep Roy, Daniel E. Ryan, Bo Curry, Magdalena Olesiak, Madhurima Singh, David Taussig, Ryan McCaffrey, Chong Wing Yung, Douglas J. Dellinger, Xuan Vuong, Smruti M Phadnis, Jeffrey R. Sampson
Publikováno v:
Nucleic Acids Research
CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly matched targets. We have developed a novel approach for improving
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5983ffc5eb2b92f101efcc6c1144cd11
http://europepmc.org/articles/PMC5778453
http://europepmc.org/articles/PMC5778453
Autor:
Subhadeep Roy, Rosa Bacchetta, Rasmus O. Bak, Matthew H. Porteus, Benjamin D. Lunstad, Anya Tsalenko, Ayal Hendel, Israel Steinfeld, Joseph T. Clark, Laurakay Bruhn, Robert J. Kaiser, Douglas J. Dellinger, Alec B. Wilkens, Daniel E. Ryan, Andrew Kennedy
Publikováno v:
Hendel, A, Bak, R O, Clark, J T, Kennedy, A B, Ryan, D E, Roy, S, Steinfeld, I, Lunstad, B D, Kaiser, R J, Wilkens, A B, Bacchetta, R, Tsalenko, A, Dellinger, D, Bruhn, L & Porteus, M H 2015, ' Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells ', Nature Biotechnology . https://doi.org/10.1038/nbt.3290
CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23cc8fdf1bd5b44d01aab22d286377be
https://pure.au.dk/portal/da/publications/chemically-modified-guide-rnas-enhance-crisprcas-genome-editing-in-human-primary-cells(d66dc88c-fd1b-4cde-a6a1-c8a8cc221e53).html
https://pure.au.dk/portal/da/publications/chemically-modified-guide-rnas-enhance-crisprcas-genome-editing-in-human-primary-cells(d66dc88c-fd1b-4cde-a6a1-c8a8cc221e53).html
Publikováno v:
RNA. 8:1011-1033
The 5' and 3' domains of yeast U6 snRNA contain sequences that are thought to be important for binding to Prp24 and Lsm proteins. By extensive mutational analysis of yeast U6 snRNA, we confirmed that the 3' terminal uridine tract of U6 snRNA is impor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::545e030e088a5f1273365880b092ff50
https://doi.org/10.1017/s1355838202026092
https://doi.org/10.1017/s1355838202026092
Publikováno v:
Inorganic Chemistry. 36:764-769
Pyridine and CO adducts of RuIIOETPP (OETPP = 2,3,7,8,12,13,17,18-octaethyl-5,10,15,20-tetraphenylporphinato) have been prepared and characterized by absorption and resonance Raman (RR) spectroscopy. Ru → porphyrin back-bonding is evident from the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9689ec28444cb2ef64afc196ca65dc4b
https://doi.org/10.1021/ic960773h
https://doi.org/10.1021/ic960773h
Autor:
Daniel E. Ryan
Publikováno v:
Tetrahedron Letters. 37:6089-6092
Syntheses of concave spacers ( 9, 27 ) proceeded via stereoselective Diels-Alder reactions. Successful preparation of asymmetrical spacer 27 relied on regioselective protection of mutual precursor 7 .
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::be33f307245d7f4fd16e732765dea42e
https://doi.org/10.1016/0040-4039(96)01299-3
https://doi.org/10.1016/0040-4039(96)01299-3
Publikováno v:
Biochemistry. 35:8640-8650
Tunichromes are yellow, polyphenolic tripeptides prevalent in blood cells of tunicates (suborders phlebobranchia and stolidobranchia). Spectrophotometric studies of reactions between tunichrome Mm-1 and VV or VIV ions were conducted in vitro in vario
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2528ef4fdd8262d7f2c03b758fd0cc0
https://doi.org/10.1021/bi9521506
https://doi.org/10.1021/bi9521506