Zobrazeno 1 - 10
of 592
pro vyhledávání: '"Daniel E. Koshland"'
Autor:
Daniel E. Koshland
Publikováno v:
Annals of the New York Academy of Sciences. 103:630-642
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fd50d9d9ecbd547dd12f1a0679fa732
https://doi.org/10.1111/j.1749-6632.1963.tb53723.x
https://doi.org/10.1111/j.1749-6632.1963.tb53723.x
Publikováno v:
Biochemistry. 43:13648-13656
A series of mutants of chymotrypsin inhibitor 2 (CI2), at residues that interact with the inhibited enzyme subtilisin BPN', were studied to determine the relative importance of intermolecular contacts on either side of the scissile bond. Mutants were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::632852739cfdd218fc8cb470b6418bfd
https://doi.org/10.1021/bi048797k
https://doi.org/10.1021/bi048797k
Publikováno v:
Science. 300:976-980
Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligandtransporter complexes have previously been unavailable. We obtained x-ray crystallographic structures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::614b50e0faf59a51ef97839bd0fe55fb
https://doi.org/10.1126/science.1083137
https://doi.org/10.1126/science.1083137
Publikováno v:
Biochemistry. 42:6484-6492
A synthetic cyclic peptide, reported to be a tight-binding inhibitor of serine proteases, is instead found to be a good substrate, as is the linear peptide of the same sequence. Both of the peptides, designed to mimic the binding loop of chymotrypsin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4f7ee58e3ab50268c1275779f20adaa
https://doi.org/10.1021/bi034275d
https://doi.org/10.1021/bi034275d
Publikováno v:
Journal of Biological Chemistry. 277:9255-9261
In this study we have examined how unnatural sialic acids can alter polysialic acid expression and influence the adhesive properties of the neural cell adhesion molecule (NCAM). Unnatural sialic acids are generated by metabolic conversion of syntheti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dfd21357065661ef145625cc98986d8
https://doi.org/10.1074/jbc.m111619200
https://doi.org/10.1074/jbc.m111619200
Autor:
Edward W. Yu, Daniel E. Koshland
Publikováno v:
Proceedings of the National Academy of Sciences. 98:9517-9520
The problem of the propagation of conformational changes over long distances or through a closely packed protein is shown to fit a model of a ligand-induced conformational change between two protein states selected by evolution. Moreover, the kinetic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32f3e12085352607bd6ddb0e623a6244
https://doi.org/10.1073/pnas.161239298
https://doi.org/10.1073/pnas.161239298
Publikováno v:
Journal of Molecular Biology. 295:377-385
Isocitrate dehydrogenase catalyses the two step, acid base, oxidative decarboxylation of isocitrate to α-ketoglutarate. Lysine 230 was suggested to act as proton donor based on geometry and spatial proximity to isocitrate. To clarify further the rol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4e0d727bc4c0ae158afb888d8921dd51
https://doi.org/10.1006/jmbi.1999.3195
https://doi.org/10.1006/jmbi.1999.3195
Publikováno v:
Protein Engineering, Design and Selection. 12:863-872
The Escherichia coli aspartate receptor is a dimer with two transmembrane sequences per monomer that connect a periplasmic ligand binding domain to a cytoplasmic signaling domain. The method of 'hydrophobic-biased' random mutagenesis, that we describ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83cb69c563677ed5ca18195ead88685c
https://doi.org/10.1093/protein/12.10.863
https://doi.org/10.1093/protein/12.10.863
Publikováno v:
Science. 285:1751-1754
To characterize the mechanism by which receptors propagate conformational changes across membranes, nitroxide spin labels were attached at strategic positions in the bacterial aspartate receptor. By collecting the electron paramagnetic resonance spec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5c406faded6ae162b09ee8dbdd79215
https://doi.org/10.1126/science.285.5434.1751
https://doi.org/10.1126/science.285.5434.1751
Publikováno v:
Nature Structural Biology. 5:891-897
The structure of a rate-limited product complex formed during a single initial round of turnover by isocitrate dehydrogenase has been determined. Photolytic liberation of either caged substrate or caged cofactor and Laue X-ray data collection were us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56dd2910414dca8b8dc151511202ffbc
https://doi.org/10.1038/2331
https://doi.org/10.1038/2331