Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Daniel DeMonte"'
Autor:
Sang Hak Lee, Chaoyi Jin, En Cai, Pinghua Ge, Yuji Ishitsuka, Kai Wen Teng, Andre A de Thomaz, Duncan Nall, Murat Baday, Okunola Jeyifous, Daniel Demonte, Christopher M Dundas, Sheldon Park, Jary Y Delgado, William N Green, Paul R Selvin
Publikováno v:
eLife, Vol 6 (2017)
Externí odkaz:
https://doaj.org/article/567e1bdae8b74f92ab6285eca5d9df67
Autor:
Sang Hak Lee, Chaoyi Jin, En Cai, Pinghua Ge, Yuji Ishitsuka, Kai Wen Teng, Andre A de Thomaz, Duncan Nall, Murat Baday, Okunola Jeyifous, Daniel Demonte, Christopher M Dundas, Sheldon Park, Jary Y Delgado, William N Green, Paul R Selvin
Publikováno v:
eLife, Vol 6 (2017)
Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR trafficking outside of, and
Externí odkaz:
https://doaj.org/article/671f3c4ec2474b219f8b84c8994f00b7
Autor:
Lawrence S. Evans, Katherine E. Lewis, Daniel DeMonte, Janhavi G. Bhandari, Logan B. Garrett, Joseph L. Kuijper, Daniel Ardourel, Martin F. Wolfson, Susan Debrot, Sherri Mudri, Kayla Kleist, Luana L. Griffin, LuAnne Hebb, Russell J. Sanderson, NingXin Wang, Michelle Seaberg, Allison G. Chunyk, Jing Yang, Youji Hong, Zahra Maria, David J. Messenheimer, Pamela M. Holland, Stanford L. Peng, Mark W. Rixon, Stacey R. Dillon
Publikováno v:
Arthritis & Rheumatology.
Publikováno v:
TECHNOLOGY. :152-158
Because streptavidin is a homotetramer, it can bind multiple biotinylated ligands and cause target aggregation. To allow biotin detection without clustering, we previously engineered monomeric streptavidin (mSA) that is structurally similar to a sing
Publikováno v:
Applied Biochemistry and Biotechnology. 177:1137-1151
Inteins are protein segments embedded in frame within a precursor sequence that catalyze a self-excision reaction and ligate the flanking sequences with a standard peptide bond. Split inteins are expressed as two separate polypeptide fragments and tr
Autor:
En Cai, Jary Y. Delgado, Okunola Jeyifous, Yuji Ishitsuka, Pinghua Ge, Christopher M. Dundas, Andre A. de Thomaz, Sang Hak Lee, Paul R. Selvin, William N. Green, Daniel Demonte, Kai Wen Teng, Sheldon Park, Murat Baday, Duncan L. Nall, Chaoyi Jin
Publikováno v:
eLife
eLife, Vol 6 (2017)
eLife, Vol 6 (2017)
Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR trafficking outside of, and
Autor:
Jary Y. Delgado, Andre A. de Thomaz, Christopher M. Dundas, Paul R. Selvin, En Cai, Daniel Demonte, Pinghua Ge, Okunola Jeyifous, Sang Hak Lee, William N. Green, Kai Wen Teng, Duncan L. Nall, Sheldon Park, Chaoyi Jin, Murat Baday, Yuji Ishitsuka
Publikováno v:
eLife, Vol 6 (2017)
eLife
eLife
Forgetting is a common experience in our everyday life. Yet much remains unknown about how we remember, and about why our memories sometimes fail us. The brain contains 80 to 100 billion nerve cells or neurons, which communicate with one another at j
Autor:
Sang Hak Lee, Chaoyi Jin, En Cai, Pinghua Ge, Yuji Ishitsuka, Kai Wen Teng, Andre A de Thomaz, Duncan Nall, Murat Baday, Okunola Jeyifous, Daniel Demonte, Christopher M Dundas, Sheldon Park, Jary Y Delgado, William N Green, Paul R Selvin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1bd9d3d2e02faa3163a4a0e078507cfe
https://doi.org/10.7554/elife.27744.036
https://doi.org/10.7554/elife.27744.036
Publikováno v:
Applied Microbiology and Biotechnology. 98:6285-6295
We recently reported the engineering of monomeric streptavidin (mSA) for use in monomeric detection of biotinylated ligands. Although mSA can be expressed functionally on the surface of mammalian cells and yeast, the molecule does not fold correctly
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 81:1621-1633
We recently reported the engineering of monomeric streptavidin, mSA, corresponding to one subunit of wild type (wt) streptavidin tetramer. The monomer was designed by homology modeling, in which the streptavidin and rhizavidin sequences were combined