Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Daniel A. Plymire"'
Autor:
Tabitha Emde, Raquel Bromberg, Yirui Guo, Maciej Puzio, Zbyszek Otwinowski, Daniel A. Plymire, Dominika Borek
The His-tag is a widely used affinity tag that facilitates purification by means of affinity chromatography of recombinant proteins for functional and structural studies. We show here that His-tag presence affects how coproheme decarboxylase interact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::33cab5a2d58da42eb160b71a3f68e07a
https://doi.org/10.1101/2020.09.22.309005
https://doi.org/10.1101/2020.09.22.309005
Autor:
Zbyszek Otwinowski, Yirui Guo, Tabitha Emde, Dominika Borek, Bradley Quade, Daniel A. Plymire, Raquel Bromberg
Publikováno v:
Acta Crystallographica Section A Foundations and Advances. 77:a197-a197
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e9ecbd6ac4336b682288b5b247fb24e0
https://doi.org/10.1107/s0108767321098020
https://doi.org/10.1107/s0108767321098020
Myelin basic protein (MBP) plays an important structural and functional role in the neuronal myelin sheath. Translated MBP exhibits extreme microheterogeneity with numerous alternative splice variants (ASVs) and post-translational modifications (PTMs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b216fbb5c33292b78a77afc964e7fd90
https://europepmc.org/articles/PMC5735417/
https://europepmc.org/articles/PMC5735417/
Publikováno v:
Analytical Chemistry. 85:10597-10604
We report novel ligand binding assay (LBA) surface modalities that permit plasma protease catalytic efficiency (kcat/km) determination by MALDI-TOF MS without the use of liquid chromatography or internal standards such as chemical or metalized labels
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80084e128bf3a36ec5c2df982fabfba6
https://doi.org/10.1021/ac402739z
https://doi.org/10.1021/ac402739z
Autor:
Steven M. Patrie, Neal M. Alto, James M. Ertelt, Sing Sing Way, Bethany A. Weigele, Andrey S. Selyunin, Nikolay Burnaevskiy, Daniel A. Plymire, Thomas G. Fox
Publikováno v:
Nature. 496:106-109
An irreversible mechanism of protein demyristoylation catalysed by invasion plasmid antigen J (IpaJ), a Shigella flexneri type III effector protein with cysteine protease activity, is described. Nearly one per cent of eukaryotic proteins are modified
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c681d6a7bb20c521f79d5fc6b967bc42
https://doi.org/10.1038/nature12004
https://doi.org/10.1038/nature12004
Autor:
Michael J. Roth, Roger Robbins, John R. Corbett, Steven M. Patrie, Junmei Zhang, Erica M. Maresh, Daniel A. Plymire
Publikováno v:
ACS Applied Materials & Interfaces. 5:6-10
Immunoassays are employed in academia and the healthcare and biotech industries for high-throughput, quantitative screens of biomolecules. We have developed monolayer-based immunoassays for MALDI-TOF MS. To improve parallelization, we adapted the wor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdbbaeb4f1910c25c8be0cb81e66cc4e
https://doi.org/10.1021/am3024756
https://doi.org/10.1021/am3024756
Autor:
Daniel A. Plymire, Carl A. Hubel, James M. Roberts, Robert W. Powers, David C. Sogin, Don M. Laird, Arun Jeyabalan, Dominick L. Pucci, Saul A. Datwyler, Robin E. Gandley
Publikováno v:
Hypertension. 60:239-246
Preeclampsia is a heterogeneous syndrome affecting 3% to 5% of all pregnancies. An imbalance of the antiangiogenic and proangiogenic factors, soluble receptor fms-like tyrosine kinase 1 and placental growth factor (PGF), is thought to contribute to t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ec243d417e0feb336849180fb3cccf43
https://doi.org/10.1161/hypertensionaha.112.191213
https://doi.org/10.1161/hypertensionaha.112.191213
Autor:
Carl A. Hubel, Lia R. Edmunds, Vivek Verma, Daniel A. Plymire, Robert W. Powers, Patrizia Luppi
Publikováno v:
Reproductive Sciences. 17:643-652
Circulating endothelial progenitor cells (EPCs) may contribute to vascular endothelial cell homeostasis, and low levels of these cells are predictive of cardiovascular disease. We hypothesized that circulating EPCs increase in number during uncomplic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13d1f34565ea51a96745b8866c81f4b3
https://doi.org/10.1177/1933719110366164
https://doi.org/10.1177/1933719110366164
Autor:
Audrey N. Chang, Daniel A. Plymire, Michael J. Roth, Benjamin Greenberg, John R. Corbett, Junmei Zhang, Steven M. Patrie
Publikováno v:
Analytical chemistry. 85(21)
Top-down mass spectrometry (MS) has emerged as a powerful complement to peptide-based proteomics. Despite advancements, the field has had limited application to clinical proteomics investigations due to the complexity and poor dynamic range of chroma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f047a9b966581fbefbff9413de8dd91
https://pubmed.ncbi.nlm.nih.gov/24074297
https://pubmed.ncbi.nlm.nih.gov/24074297
Publikováno v:
Proteomics. 14(10)
Lipocalin-type prostaglandin D-synthase (L-PGDS) in cerebrospinal fluid contributes to the maturation and maintenance of the CNS. L-PGDS PTMs may contribute to pathobiology of different CNS diseases, but methods to monitor its proteoforms are limited
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40a9514a135ecdbee93b6e2bf7879c81
https://pubmed.ncbi.nlm.nih.gov/24678018
https://pubmed.ncbi.nlm.nih.gov/24678018