Gene knock-outs in human CD34+ hematopoietic stem and progenitor cells and in the human immune system of mice.

Autor: Daniel A Kuppers, Jonathan Linton, Sergio Ortiz Espinosa, Kelly M McKenna, Anthony Rongvaux, Patrick J Paddison

Publikováno v: PLoS ONE, Vol 18, Iss 6, p e0287052 (2023)

Human CD34+ hematopoietic stem and progenitor cells (HSPCs) are a standard source of cells for clinical HSC transplantations as well as experimental xenotransplantation to generate "humanized mice". To further extend the range of applications of thes

Externí odkaz: https://doaj.org/article/c40eaaf9412747dea3045397d9b776a9

Functional genomic analysis of adult and pediatric brain tumor isolates

Autor: Pia Hoellerbauer, Matt C. Biery, Sonali Arora, Yiyun Rao, Emily J. Girard, Kelly Mitchell, Pratiksha Dighe, Megan Kufeld, Daniel A. Kuppers, Jacob A. Herman, Eric C. Holland, Liliana Soroceanu, Nicholas A. Vitanza, James M. Olson, Justin R. Pritchard, Patrick J. Paddison

BackgroundAdult and pediatric tumors display stark differences in their mutation spectra and chromosome alterations. Here, we attempted to identify common and unique gene dependencies and their associated biomarkers among adult and pediatric tumor is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e88e907c9043c2c5a83a45d6372a7f63
https://doi.org/10.1101/2023.01.05.522885

N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis

Autor: Yiting Lim, Patrick J. Paddison, Housheng Hansen He, Lucas Carter, Andrea R. Lim, Ryan Basom, Jeffrey J. Delrow, Philip Corrin, Shiyan Wang, Christopher L. Plaisier, Daniel A. Kuppers, Beverly Torok-Storb, Andrew C. Hsieh, Sonali Arora

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019)
Nature Communications

Many of the regulatory features governing erythrocyte specification, maturation, and associated disorders remain enigmatic. To identify new regulators of erythropoiesis, we utilize a functional genomic screen for genes affecting expression of the ery

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28e7320aecb26108066f4722f16abe97
http://link.springer.com/article/10.1038/s41467-019-12518-6

The miR-106a~363Xpcl1 miRNA cluster induces murine T cell lymphoma despite transcriptional activation of the p27Kip1 cell cycle inhibitor

Autor: Harry C. Hwang, Daniel A. Kuppers, Lavanya Samraj, Matthew L. Fero, Thomas M. Schmitt, Bruce E. Clurman

Publikováno v: Oncotarget

// Daniel A. Kuppers 1 , Thomas M. Schmitt 1 , Harry C. Hwang 2 , Lavanya Samraj 3 , Bruce E. Clurman 1 and Matthew L. Fero 4 1 Fred Hutchinson Cancer Research Center, Seattle, Washington, USA 2 Phenopath Laboratories, Seattle, Washington, USA 3 Univ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca8ccc734b9b7019f85f06ba1cbc69e7
https://doi.org/10.18632/oncotarget.16932

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency

Autor: Julie Mathieu, Logeshwaran Somasundaram, Hannele Ruohola-Baker, Sonia B. Sidhu, Aaron M. Robitaille, A. McAlister, T. Bottorff, Damien Detraux, Randall T. Moon, Filippo Artoni, R. D. Hawkins, Daniel A. Kuppers, Patrick J. Paddison, Amy Ferreccio, Carol B. Ware, Christopher Cavanaugh, Shiri Levy, Yuliang Wang, Stephanie L. Battle

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Mathieu, J, Detraux, D, Kuppers, D, Wang, Y, Cavanaugh, C, Sidhu, S, Levy, S, Robitaille, A M, Ferreccio, A, Bottorff, T, McAlister, A, Somasundaram, L, Artoni, F, Battle, S, Hawkins, R D, Moon, R T, Ware, C B, Paddison, P J & Ruohola-Baker, H 2019, ' Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency ', Nature Communications, vol. 10, no. 1, 632, pp. 632 . https://doi.org/10.1038/s41467-018-08020-0
Nature Communications

To reveal how cells exit human pluripotency, we designed a CRISPR-Cas9 screen exploiting the metabolic and epigenetic differences between naïve and primed pluripotent cells. We identify the tumor suppressor, Folliculin(FLCN) as a critical gene requi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c851627584c445206f3ebbb7d19bc850
https://doi.org/10.1038/s41467-018-08020-0