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G Protein–Coupled Estrogen Receptor Production Using an Escherichia coli Cell‐Free Expression System
Publikováno v:
Current Protocols in Protein Science. 97
Heterologous expression of the G protein-coupled estrogen receptor (GPER) comes with a suite of challenges intrinsic to membrane proteins. This receptor's low expression levels and tendency to form insoluble aggregates in Escherichia coli and yeast m