Preparation of Leptin Antagonists by Site-Directed Mutagenesis of Human, Ovine, Rat, and Mouse Leptin's Site III

Autor: Leonora Niv-Spector, Gili Solomon, Jean Djiane, Arieh Gertler, Isabelle Callebaut, Dana Gonen-Berger

Publikováno v: Annals of the New York Academy of Sciences. 1091:531-539

Six muteins of human, ovine, rat, and mouse leptins mutated to Ala in amino acids 39-41 or 39-42 were prepared by site-directed mutagenesis of the putative site III, which does not affect binding but is necessary for receptor activation, then express

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a324cd720f653a7f3d2aca150d1341df
https://doi.org/10.1196/annals.1378.094

Identification of the hydrophobic strand in the A–B loop of leptin as major binding site III: implications for large-scale preparation of potent recombinant human and ovine leptin antagonists

Autor: Krishnan V. Ramanujan, Eugene E. Gussakovsky, Dana Gonen-Berger, Jean Djiane, Eva Biener, Leonora Niv-Spector, Yackir Benomar, Arieh Gertler, Brian Herman, Isabelle Gourdou, Mohammed Taouis, Isabelle Callebaut

Publikováno v: Biochemical Journal
Biochemical Journal, 2005, 391, pp.221-230. ⟨10.1042/BJ20050457⟩
Biochemical Journal, 2005, 391 (2), pp.221-230. ⟨10.1042/BJ20050457⟩
Biochemical Journal, Portland Press, 2005, 391, pp.221-230. ⟨10.1042/BJ20050457⟩
Biochemical Journal, Portland Press, 2005, 391 (2), pp.221-230. ⟨10.1042/BJ20050457⟩

International audience; Interaction of leptin with its receptors resembles that of interleukin-6 and granulocyte colony-stimulating factor, which interact with their receptors through binding sites I–III. Site III plays a pivotal role in receptors'

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c4f254bd19692820414e64e3a2c7558
https://hal.science/hal-00020373