Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Dan Y. Wu"'
Publikováno v:
Methods. 2:42-48
A rapid, non-radioactive approach to the diagnosis of sickle cell anemia is described based on an allele specific polymerase chain reaction (ASPCR) in which the 3'-terminal nucleotide of one of the primers of the primer set forms a match with one all
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a682c840b54e2a0e9e068aa74760608
https://doi.org/10.1016/s1046-2023(05)80124-0
https://doi.org/10.1016/s1046-2023(05)80124-0
Publikováno v:
DNA and cell biology. 10(3)
The polymerase chain reaction (PCR) is most effectively performed using a thermostable DNA polymerase such as that isolated from Thermus aquaticus. Since temperature and oligonucleotide length are known to control the specificity of oligonucleotide h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78c0bef7db278344c10c77be349d4af5
https://pubmed.ncbi.nlm.nih.gov/2012681
https://pubmed.ncbi.nlm.nih.gov/2012681
Publikováno v:
DNA. 8:135-142
Using oligonucleotide hybridization, single and multiple nucleotide differences between alleles were detected directly in genomic DNA without electrophoretic separation. The DNA was immobilized in depressions in an agarose gel (in situ dots) and hybr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0a568c6275f107f67c69a2cfa099204
https://doi.org/10.1089/dna.1.1989.8.135
https://doi.org/10.1089/dna.1.1989.8.135
Publikováno v:
Proceedings of the National Academy of Sciences. 86:2757-2760
A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc43659839e0073548750b2aac7f1256
https://doi.org/10.1073/pnas.86.8.2757
https://doi.org/10.1073/pnas.86.8.2757