Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Dan Sphar"'
Autor:
Paula King, Long K Pham, Shannon Waltz, Dan Sphar, Robert T Yamamoto, Douglas Conrad, Randy Taplitz, Francesca Torriani, R Allyn Forsyth
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0160124 (2016)
We describe the sampling of sixty-three uncultured hospital air samples collected over a six-month period and analysis using shotgun metagenomic sequencing. Our primary goals were to determine the longitudinal metagenomic variability of this environm
Externí odkaz:
https://doaj.org/article/d38ee2d84ab246f6b67429e062285f09
Autor:
Guohong Liu, Christopher Q Weston, Long K Pham, Shannon Waltz, Helen Barnes, Paula King, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0146064 (2016)
We describe continuing work to develop restriction endonucleases as tools to enrich targeted genomes of interest from diverse populations. Two approaches were developed in parallel to segregate genomic DNA based on cytosine methylation. First, the me
Externí odkaz:
https://doaj.org/article/32f679157d4e4f4b9fe604d53ad91ec9
Autor:
Paula King, Long K Pham, Shannon Waltz, Dan Sphar, Robert T Yamamoto, Douglas Conrad, Randy Taplitz, Francesca Torriani, R Allyn Forsyth
Publikováno v:
PLoS ONE, Vol 11, Iss 12, p e0169376 (2016)
[This corrects the article DOI: 10.1371/journal.pone.0160124.].
Externí odkaz:
https://doaj.org/article/1b1c659241614518811aa8cada294f80
Autor:
Helen E Barnes, Guohong Liu, Christopher Q Weston, Paula King, Long K Pham, Shannon Waltz, Kimberly T Helzer, Laura Day, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth
Publikováno v:
PLoS ONE, Vol 9, Iss 10, p e109061 (2014)
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows spe
Externí odkaz:
https://doaj.org/article/f4589789518941dbb5e98d04134b80f0
Autor:
Helen E. Barnes, Paula King, Long K. Pham, Guohong Liu, R. Allyn Forsyth, Robert T. Yamamoto, Dan Sphar, Christopher Q. Weston, Shannon Waltz
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0146064 (2016)
PLoS ONE
PLoS ONE
We describe continuing work to develop restriction endonucleases as tools to enrich targeted genomes of interest from diverse populations. Two approaches were developed in parallel to segregate genomic DNA based on cytosine methylation. First, the me
Autor:
Shannon Waltz, Douglas Conrad, R. Allyn Forsyth, Paula King, Dan Sphar, Robert T. Yamamoto, Randy Taplitz, Long K. Pham, Francesca J. Torriani
Publikováno v:
PLoS ONE, Vol 11, Iss 12, p e0169376 (2016)
PloS one, vol 11, iss 12
PloS one, vol 11, iss 12
The Materials and Methods section does not describe the ZovaSeq pipeline used to analyze the data in sufficient detail. The attached Supporting Information file provides additional information about the version of ZovaSeq used for King et al. 2016 [1
Autor:
Long K. Pham, R. Allyn Forsyth, Robert T. Yamamoto, Douglas Conrad, Shannon Waltz, Randy Taplitz, Paula King, Francesca J. Torriani, Dan Sphar
Publikováno v:
PLoS ONE
PloS one, vol 11, iss 8
PLoS ONE, Vol 11, Iss 8, p e0160124 (2016)
PloS one, vol 11, iss 8
PLoS ONE, Vol 11, Iss 8, p e0160124 (2016)
We describe the sampling of sixty-three uncultured hospital air samples collected over a six-month period and analysis using shotgun metagenomic sequencing. Our primary goals were to determine the longitudinal metagenomic variability of this environm
Autor:
Kimberly T. Helzer, Helen E. Barnes, Dan Sphar, Christopher Q. Weston, R. Allyn Forsyth, Laura Day, Robert T. Yamamoto, Shannon Waltz, Guohong Liu, Paula King, Long K. Pham
Publikováno v:
PLoS ONE
PLoS ONE, Vol 9, Iss 10, p e109061 (2014)
PLoS ONE, Vol 9, Iss 10, p e109061 (2014)
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows spe