Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Dan F, Lazar"'
Publikováno v:
Cancer Research. 83:1428-1428
We have developed homogeneous, luminescent immunoassays for simple and rapid determination of Ki-67 and uPAR levels in 96-well format, thereby enabling efficient and quantitative assessment of proliferation and senescence in mammalian cell culture. A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2ab175624bef2fe3470d4771922d4477
https://doi.org/10.1158/1538-7445.am2023-1428
https://doi.org/10.1158/1538-7445.am2023-1428
Publikováno v:
Methods in cell biology. 165
Autophagy is an important intracellular pathway for the degradation of superfluous or harmful subcellular materials, thereby playing a critical role in the maintenance of cell health under normal and stress-related conditions. Researchers interrogati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2e0ad41d3aeaa789153d08ec06890579
https://pubmed.ncbi.nlm.nih.gov/34311870
https://pubmed.ncbi.nlm.nih.gov/34311870
Autor:
Dan F Lazar, Kevin Hsiao, Casey Sondgeroth, Julia Gilden, Kevin Kupcho, David Thompson, Martha O’Brien, James Cali
Publikováno v:
The Journal of Immunology. 206:27.25-27.25
Rapid (< 70 min), add-and-read assays for cytokine determination were developed with Lumit™ immunoassay technology. Separate antibodies labeled with complementary SmBiT or LgBiT subunits bind to their shared cytokine target, resulting in proximity-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::268c899c842ecce46e4d4874a11d6f8f
https://doi.org/10.4049/jimmunol.206.supp.27.25
https://doi.org/10.4049/jimmunol.206.supp.27.25
Autor:
Dan F Lazar, Kevin R Kupcho, Casey A Sondgeroth, Kevin Hsiao, David V Thompson, Martha A O’Brien, Julia K Gilden, James J Cali
Publikováno v:
The Journal of Immunology. 204:59.31-59.31
We have utilized NanoLuc® Binary Technology (NanoBiT®) to develop a homogeneous and rapid assay method (≤ 70 min completion time) to measure cytokines released from cells in culture without the need for sample transfer and requiring only a standa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d69bc05ed9c5da1f38ab05298ab1c73c
https://doi.org/10.4049/jimmunol.204.supp.59.31
https://doi.org/10.4049/jimmunol.204.supp.59.31
Publikováno v:
Cancer Research. 79:710-710
The structural and biological complexity of cancer spheroids make them uniquely suited to address or approximate the pharmacodynamics of new chemical entities directed at chemotherapeutic reduction of tumor masses. Unfortunately, this same complexity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7f62f6d143bf7ede574a62dfb24d3a8
https://doi.org/10.1158/1538-7445.am2019-710
https://doi.org/10.1158/1538-7445.am2019-710
Autor:
Keishi Yamauchi, Cynthia Corley Mastick, Jeffrey E. Pessin, Pedro Cuatrecasas, Matthew J. Brady, Steven B. Waters, Russell J. Wiese, Dan F. Lazar, Alan R. Saltiel
Publikováno v:
Journal of Biological Chemistry. 270:20801-20807
Insulin stimulates the activity of mitogen-activated protein kinase (MAPK) via its upstream activator, MAPK kinase (MEK), a dual specificity kinase that phosphorylates MAPK on threonine and tyrosine. The potential role of MAPK activation in insulin a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a49fa5adc5ed5c7753953f95b63c5374
https://doi.org/10.1074/jbc.270.35.20801
https://doi.org/10.1074/jbc.270.35.20801
Publikováno v:
Scopus-Elsevier
Activation of the tyrosine kinase activity of the insulin receptor by autophosphorylation leads to phosphorylation of cellular substrates on tyrosine. Thus far, the best characterized is the insulin receptor substrate (IRS) 1, which has been proposed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cfffde69461040dc7b791f090abe0d2
https://doi.org/10.1042/bj3080579
https://doi.org/10.1042/bj3080579
Publikováno v:
Biochemical and Biophysical Research Communications. 196:301-310
Naturally occurring human insulin receptor mutants Ser 1200 and Thr 1134 , and a site-directed mutant Arg 1030 overexpressed in Chinese hamster ovary (CHO) cells, bind insulin with affinities identical to wildtype receptors but are apparently kinase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a091e74fd4c076e3295a34d9eadfbf65
https://doi.org/10.1006/bbrc.1993.2249
https://doi.org/10.1006/bbrc.1993.2249
Autor:
Fedor Medzihradsky, Dan F. Lazar
Publikováno v:
Journal of Neurochemistry. 61:1135-1140
In unmodified synaptosomal brain membranes the presence of NaCl inhibited the binding to μ receptors of the tritiated opioid agonists etorphine, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol, and sufentanil by 53, 43, and 37%, respectively, and increased that of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caf88c6058bf8269c48a22aa928ca334
https://doi.org/10.1111/j.1471-4159.1993.tb03630.x
https://doi.org/10.1111/j.1471-4159.1993.tb03630.x
Autor:
Dan F. Lazar, Fedor Medzihradsky
Publikováno v:
Journal of Neurochemistry. 59:1233-1240
In synaptosomal membranes from rat and monkey brain cortex, the addition of petroselenic (18:1, cis-delta 6) acid, oleic (18:1, cis-delta 9) acid, and vaccenic (18:1, cis-delta 11) acid or their corresponding methyl esters at 0.5 mumol/mg of membrane
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aac78dca00a350e68127b09391fd1e6
https://doi.org/10.1111/j.1471-4159.1992.tb08432.x
https://doi.org/10.1111/j.1471-4159.1992.tb08432.x