Purification and initial characterization of Plasmodium falciparum K+ channels, PfKch1 and PfKch2 produced in Saccharomyces cerevisiae

Autor: Karen Molbaek, Maria Tejada, Christina Hoeier Ricke, Peter Scharff-Poulsen, Peter Ellekvist, Claus Helix-Nielsen, Nirbhay Kumar, Dan A. Klaerke, Per Amstrup Pedersen

Publikováno v: Microbial Cell Factories, Vol 19, Iss 1, Pp 1-16 (2020)

Abstract Resistance towards known antimalarial drugs poses a significant problem, urging for novel drugs that target vital proteins in the malaria parasite Plasmodium falciparum. However, recombinant production of malaria proteins is notoriously diff

Externí odkaz: https://doaj.org/article/55b4b6c89b054c8ba70fa0ad852028b0

pXOOY: A dual-function vector for expression of membrane proteins in Saccharomyces cerevisiae and Xenopus laevis oocytes.

Autor: Victoria Amstrup Vold, Sebastian Glanville, Dan Arne Klaerke, Per Amstrup Pedersen

Publikováno v: PLoS ONE, Vol 18, Iss 2, p e0281868 (2023)

On the quest for solving structures of membrane proteins by X-ray crystallography or cryo-EM, large quantities of ultra-pure protein are a paramount prerequisite. Obtaining enough protein of such high standard is not a trivial task, especially for di

Externí odkaz: https://doaj.org/article/ae4065e30cf445fb83e97168e33679d0

Structure of the human ClC-1 chloride channel.

Autor: Kaituo Wang, Sarah Spruce Preisler, Liying Zhang, Yanxiang Cui, Julie Winkel Missel, Christina Grønberg, Kamil Gotfryd, Erik Lindahl, Magnus Andersson, Kirstine Calloe, Pascal F Egea, Dan Arne Klaerke, Michael Pusch, Per Amstrup Pedersen, Z Hong Zhou, Pontus Gourdon

Publikováno v: PLoS Biology, Vol 17, Iss 4, p e3000218 (2019)

ClC-1 protein channels facilitate rapid passage of chloride ions across cellular membranes, thereby orchestrating skeletal muscle excitability. Malfunction of ClC-1 is associated with myotonia congenita, a disease impairing muscle relaxation. Here, w

Externí odkaz: https://doaj.org/article/83a7e6f4ff29469c8e807f5e8caa11cf

Saccharomyces cerevisiae as a superior host for overproduction of prokaryotic integral membrane proteins

Autor: Kirstine Calloe, Sarah Spruce Preisler, Molly Hurd, Pontus Gourdon, Casper Normann Nurup, Per Amstrup Pedersen, Zsófia Szathmáry, Anders Wiuf, Marc Friis, Kamil Gotfryd, Frederik Bühring Bjoerkskov, Dan A. Klaerke, Eva Ramos Becares, Lasse Kjaergaard

Publikováno v: Current Research in Structural Biology
Current Research in Structural Biology, Vol 3, Iss, Pp 51-71 (2021)

Integral membrane proteins (IMPs) constitute ~30% of all proteins encoded by the genome of any organism and Escherichia coli remains the first-choice host for recombinant production of prokaryotic IMPs. However, the expression levels of prokaryotic I

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16b7a81f33fd468e41366d521e9d8918
http://europepmc.org/articles/PMC8244417

Exercise-Associated Sudden Death in Finnish Standardbred and Coldblooded Trotters - A Case Series With Pedigree Analysis

Autor: Dagmar S Trachsel, Katariina Lamminpää, Pia Raistakka, Anna Mykkänen, Kirstine Calloe, Marjukka Anttila, Merete Fredholm, Dan A. Klaerke, Martti Tala, Rikke Buhl

Publikováno v: Trachsel, D S, Calloe, K, Mykkänen, A K, Raistakka, P, Anttila, M, Fredholm, M, Tala, M, Lamminpää, K, Klaerke, D A & Buhl, R 2021, ' Exercise-Associated Sudden Death in Finnish Standardbred and Coldblooded Trotters-A Case Series With Pedigree Analysis ', Journal of Equine Veterinary Science, vol. 104, 103694 . https://doi.org/10.1016/j.jevs.2021.103694

Exercise-associated sudden deaths (EASDs) are deaths occurring unexpectedly during or immediately after exercise. Sudden cardiac death (SCD) is one cause of EASD. Cardiac arrhythmias caused by genetic variants have been linked to SCD in humans. We hy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3a22cc8ef280deefb8d7b8a64b804a3
https://pubmed.ncbi.nlm.nih.gov/34416991