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Akademický článek
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Autor:
Hu, Chengchen, Wang, Kimberly, Damon, Ceylan, Fu, Yi, Ma, Tengjiao, Kratz, Lisa, Lal, Bachchu, Ying, Mingyao, Xia, Shuli, Cahill, Daniel P, Jackson, Christopher M, Lim, Michael, Laterra, John, Li, Yunqing
Publikováno v:
Neuro-Oncology; Jun2022, Vol. 24 Issue 6, p888-900, 13p
Autor:
Damon Ceylan, Scott Bailey, Taekjip Ha, Anustup Poddar, John Mallon, Jingyi Fei, Yanbo Wang, Olivia Yang, Digvijay Singh
Publikováno v:
Nature structuralmolecular biology. 25(4)
Cas9 (from Streptococcus pyogenes) in complex with a guide RNA targets complementary DNA for cleavage. Here, we developed a single-molecule FRET analysis to study the mechanisms of specificity enhancement of two engineered Cas9s (eCas9 and Cas9-HF1).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cb2ee18cac13e4c00921ccd9a7a196c
https://pubmed.ncbi.nlm.nih.gov/29622787
https://pubmed.ncbi.nlm.nih.gov/29622787
Autor:
Yanbo Wang, Taekjip Ha, Scott Bailey, Anustup Poddar, Olivia Yang, Damon Ceylan, Jingyi Fei, Digvijay Singh, John Mallon
In microbes, CRISPR-Cas systems provide adaptive immunity against invading genetic elements. Cas9 in complex with a guide-RNA targets complementary DNA for cleavage and has been repurposed for wide-ranging biological applications. New Cas9s have been
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc3fec58aa2ab93754644e147c49d790
