Výsledky vyhledávání - "Daljit S. Bahia"

The human δ opioid receptor activates Gi1α more efficiently than Go1α

Autor: Antonella Cavalli, Hyo-Eun Moon, Graeme Milligan, Marcel Hoffmann, Daljit S. Bahia, Dominique Massotte

Publikováno v: Journal of Neurochemistry. 76:1805-1813

To assess the relative capacity of the human δ opioid receptor to activate closely related G proteins, fusion proteins were constructed in which the α-subunits of either Gi1 or Go1, containing point mutations to render them insensitive to the actio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aea0fa8053985c420681899fc582c5d3
https://doi.org/10.1046/j.1471-4159.2001.00196.x

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Control of the efficiency of agonist-induced information transfer and stability of the ternary complex containing the δ opioid receptor and the α subunit of Gi1 by mutation of a receptor/G protein contact interface

Autor: Marcel Hoffmann, Antonella Cavalli, Daljit S. Bahia, Hyo-Eun Moon, Graeme Milligan

Publikováno v: Neuropharmacology. 41:321-330

Fusion proteins were constructed between the δ opioid receptor and forms of the α subunit of Gi1 in which cysteine351 was mutated to a range of amino acids. GDP reduced the binding of the agonist [ 3 H ] DADLE but not the antagonist [ 3 H ] naltrin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1f89d7b4e840113993a24127a6ee05c
https://doi.org/10.1016/s0028-3908(01)00076-4

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Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity of RGS4

Autor: Antonella Cavalli, Richard J. Ward, Teresa L.Z. Jones, Daljit S. Bahia, Kirk M. Druey, Graeme Milligan, Nana Sartania

Publikováno v: Journal of neurochemistry. 85(5)

Agonists stimulated high-affinity GTPase activity in membranes of HEK293 cells following coexpression of the alpha 2A-adrenoceptor and a pertussis toxin-resistant mutant of Go1 alpha. Enzyme kinetic analysis of Vmax and Km failed to detect regulation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::942ea9781d3eddd98b13f151353f466b
https://pubmed.ncbi.nlm.nih.gov/12753087

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Modulation of relative intrinsic activity of agonists at the alpha-2A adrenoceptor by mutation of residue 351 of G protein gi1alpha

Autor: Graeme Milligan, Daljit S. Bahia, Vicky N. Jackson

Publikováno v: Molecular pharmacology. 55(2)

Compared with epinephrine, the relative intrinsic activity of a series of partial agonists to activate fusion proteins between the porcine alpha-2A adrenoceptor and the alpha-subunit of Gi1 was reduced after a single-point mutation (Cys351Gly) in the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89360a9332c402dfc45d44fcaf5aadc5
https://pubmed.ncbi.nlm.nih.gov/9927608

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Selective activation of a chimeric Gi1/Gs G protein alpha subunit by the human IP prostanoid receptor: analysis using agonist stimulation of high affinity GTPase activity and [35S]guanosine-5'-O-(3-thio)triphosphate binding

Autor: Daljit S. Bahia, Stephen Rees, Graeme Milligan, Chee Wai Fong

Publikováno v: Molecular pharmacology. 54(2)

A FLAG-tagged form of the human IP prostanoid receptor was expressed stably in HEK 293 cells. This bound [3H]iloprost with high affinity and stimulated cAMP production when exposed to agonist. Iloprost produced weak stimulation of GTPase activity and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::908b94fcc3d094a9c292f21778aa5ac1
https://pubmed.ncbi.nlm.nih.gov/9687565

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