Zobrazeno 1 - 10
of 83
pro vyhledávání: '"Daizo Koga"'
Publikováno v:
Journal of Asia-Pacific Entomology. 15:397-400
This study investigated the potential of a fluorescein-5 isothiocyanate conjugated-chitin-binding domain (FITC-CBD) probe to detect chitinous materials in the peritrophic membrane (PM) structure of the Japanese pine sawyer beetle, Monochamus alternat
Autor:
Katsuhiko Endo, Hiroyuki Tado, Khondkar Ehteshamul Kabir, Akira Yamanaka, Shuhei Tanaka, Hiroyuki Sugimoto, Daizo Koga
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 70:219-229
Bombyx mori chitinase (Bm-CHI), with a molecular mass of 75 kDa, was investigated on the possibility that it can serve as a biocontrol agent against the adult Japanese pine sawyer (JPS), Monochamus alternatus (Coleoptera: Cerambycidae). Oral ingestio
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 68:1518-1524
Genomic DNA encoding a class IV chitinase was cloned from yam (Dioscorea opposita Thunb) leaves in previous research (Biosci. Biotechnol. Biochem., 68, 1508-1517 (2004)). But this chitinase had an additional sequence composed of eight amino acids (a
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 68:1338-1344
Kinetic analysis was done on the 46-kDa chitinase (EC 3.2.1.14) purified from the stomach of red sea bream, Pagrus major, using glycolchitin and N-acetylchitooligosaccharides (GlcNAc(n), n=2-6) as substrates. High activity was observed at two pHs, su
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 67:221-224
We investigated whether a plant chitinase can be used as a biocontrol agent instead of chemical fungicides by spraying chitinase E (family 19; class IV) from a yam (Dioscorea opposita Thunb) alone or together with beta-1,3-glucanase directly onto the
Autor:
Daizo Koga, Babiker M. A. Abdel-Banat
Publikováno v:
Journal of Biological Chemistry. 277:30524-30534
The gene of chitinase in the silkworm, Bombyx mori, generates four mRNA products by alternative splicing. Nucleotide sequences of the entire gene for chitinase and respective cDNAs demonstrate that the pre-mRNA undergoes alternative splicing at both
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 66:1119-1122
The hydrolytic reactions of Bombyx mori 65-kDa chitinase with the short substrates, N-acetyl-chitooligosaccharides, were analyzed by HPLC. Analysis of the hydrolyzed products showed that the newly produced oligosaccharides are all beta anomers, sugge
Autor:
Takayuki Ishimaru, Akira Saito, Daizo Koga, Shuhei Tanaka, Yusuke Matsuda, Muhammad Ali Akond, Ken Iwai, Jun Kobayashi, Akio Kato
Publikováno v:
Bioscience, biotechnology, and biochemistry. 78(2)
A yam (Dioscorea opposita Thunb) class IV chitinase, whose genomic DNA was cloned by Mitsunaga et al. (2004), was produced by the recombinant Pichia pastoris X-33 in high yields such as 66 mg/L of culture medium. The chitinase was purified by column
Autor:
Yasuyuki Arakane, Daizo Koga, Hironori Hoshika, Chika Fujiya-Tsujimoto, Natsumi Kawashima, Yuji Sasaki
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 64:723-730
To evaluate the anti-pathogen activity of chitinases, we developed a new method for measuring the lytic activity, and investigated the correlation of the lytic activity with the enzymatic properties by using four chitinase isozymes, Chitinases E, F,
Publikováno v:
Insect Biochemistry and Molecular Biology. 29:537-547
The 54 kDa protein that was suggested to be processed from the 65 kDa and 88 kDa chitinases of Bombyx mori [Koga et al., Insect Biochem. Mol. Biol. 27, 757‐767 (1997)] was purified and proved to be a third chitinase (EC 3.2.1.14). This chitinase wa