Zobrazeno 1 - 10
of 48
pro vyhledávání: '"Daiyu ITO"'
Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube
Externí odkaz:
https://doaj.org/article/c4a958b296f949a7815fb97e7624666a
Publikováno v:
The Journal of Reproduction and Development, Vol 69, Iss 4, Pp 198-205 (2023)
Although freeze-drying sperm can save space, reduce maintenance costs, and facilitate the transportation of genetic samples, the current method requires breakable, custom-made, and expensive glass ampoules. In the present study, we developed a simple
Externí odkaz:
https://doaj.org/article/e66f5b0118a8496ab3d36164ee93d6d2
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-8 (2022)
Abstract Mouse cloning by nuclear transfer using freeze-drying (FD) somatic cells is now possible, but the success rate is significantly lower than that of FD spermatozoa. Because spermatozoa, unlike somatic cells, are haploid cells with hardened nuc
Externí odkaz:
https://doaj.org/article/4cdbbf85b7fe43fcabd41442eae8b7c0
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-9 (2022)
The development of safe preservation methods for genetic resources is important. Here, the authors successfully produce cloned mice from freeze-dried somatic cells, demonstrating the possibility of safe and low-cost preservation of genetic resources.
Externí odkaz:
https://doaj.org/article/a9fbc47c206440e687a4263bcb2a5024
Autor:
Natsuki USHIGOME, Sayaka WAKAYAMA, Kango YAMAJI, Daiyu ITO, Masatoshi OOGA, Teruhiko WAKAYAMA
Publikováno v:
The Journal of Reproduction and Development, Vol 68, Iss 4, Pp 262-270 (2022)
Freeze-dried sperm (FD sperm) are of great value because they can be stored at room temperature for long periods of time, However, the birth rate of offspring derived from FD sperm is low and the step in the freeze-drying process particularly respons
Externí odkaz:
https://doaj.org/article/1bfb517fa31e4688ac0109d6313d0cbb
Autor:
Erika HAYASHI, Sayaka WAKAYAMA, Daiyu ITO, Ayumi HASEGAWA, Keiji MOCHIDA, Masatoshi OOGA, Atsuo OGURA, Teruhiko WAKAYAMA
Publikováno v:
The Journal of Reproduction and Development, Vol 68, Iss 2, Pp 118-124 (2022)
Mammalian embryos are most commonly cryopreserved in liquid nitrogen; however, liquid nitrogen is not available in special environments, such as the International Space Station (ISS), and vitrified embryos must be stored at −80°C. Recently, the hi
Externí odkaz:
https://doaj.org/article/f87a18adb9764d4a96fb8d8d79088e4e
Autor:
Daiyu Ito, Teruhiko Wakayama
Publikováno v:
STAR Protocols, Vol 2, Iss 4, Pp 100933- (2021)
Summary: The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plasti
Externí odkaz:
https://doaj.org/article/50a1d49e772549db93ceae839ebd4537
Publikováno v:
iScience, Vol 24, Iss 8, Pp 102815- (2021)
Summary: Freeze-drying techniques allow the preservation of mammalian spermatozoa without using liquid nitrogen. However, the current method requires the use of glass ampoules, which are breakable, expensive, and bulky to store or transport. In this
Externí odkaz:
https://doaj.org/article/1426067945e4475b8498fbce1e38fb3a
Autor:
Daiyu ITO, Sayaka WAKAYAMA, Yuko KAMADA, Ikue SHIBASAKI, Satoshi KAMIMURA, Masatoshi OOGA, Teruhiko WAKAYAMA
Publikováno v:
The Journal of Reproduction and Development, Vol 65, Iss 4, Pp 353-359 (2019)
Freeze-drying of spermatozoa is a convenient and safe method to preserve mammalian genetic material without the use of liquid nitrogen or a deep freezer. However, freeze-dried spermatozoa (FD sperm) are not frequently used because of the low success
Externí odkaz:
https://doaj.org/article/1b4d0767808d47d38d2016dd72ed492e
Publikováno v:
PLoS ONE, Vol 16, Iss 12, p e0260645 (2021)
Conventional in vitro culture and manipulation of mouse embryos require a CO2 incubator, which not only increases the cost of performing experiments but also hampers the transport of embryos to the other laboratories. In this study, we established an
Externí odkaz:
https://doaj.org/article/fde11b084735488e83f90d28b831723f