Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Daisy, Dewitte"'
Autor:
Daisy Dewitte, David Bourry, Frans Borremans, José C. Martins, Stefaan Rossenu, Christophe Ampe, Marleen Van Troys, Joël Vandekerckhove, W Vermeulen, Marc Goethals
Publikováno v:
Journal of Molecular Biology. 359:1277-1292
The HeadPiece (HP) domain, present in several F-actin-binding multi-domain proteins, features a well-conserved, solvent-exposed PXWK motif in its C-terminal subdomain. The latter is an autonomously folding subunit comprised of three alpha-helices org
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76aa118e0efb35216dd05f309cdc07ea
https://doi.org/10.1016/j.jmb.2006.04.042
https://doi.org/10.1016/j.jmb.2006.04.042
Autor:
Daisy Dewitte, Joël Vandekerckhove, Natalie De Ruyck, Kanako Ono, Christophe Ampe, Shoichiro Ono, Veronique Jonckheere, Marleen Van Troys
Publikováno v:
Molecular Biology of the Cell. 15:4735-4748
Generating specific actin structures via controlled actin polymerization is a prerequisite for eukaryote development and reproduction. We here report on an essential Caenorhabditis elegans protein tetraThymosinbeta expressed in developing neurons and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f10d6f82be1eb91f1d1ae5ed2ccb67b2
https://doi.org/10.1091/mbc.e04-03-0225
https://doi.org/10.1091/mbc.e04-03-0225
Autor:
Daisy Dewitte, Stefaan Rossenu, Marleen Van Troys, Joël Vandekerckhove, Danny Peelaers, Shirley Leyman, Christophe Ampe, Veronique Jonckheere
Publikováno v:
Journal of Biological Chemistry. 278:16642-16650
We propose phage display combined with enzyme-linked immunosorbent assay as a tool for the systematic analysis of protein-protein interactions by investigating the binding behavior of variants to a partner protein. Via enzyme-linked immunosorbent ass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b68d19bb96e46dce7ab2cc28478f2965
https://doi.org/10.1074/jbc.m208311200
https://doi.org/10.1074/jbc.m208311200
Autor:
Marleen Van Troys, Daisy Dewitte, M Goethals, Christophe Ampe, Jean-Luc Verschelde, Joël Vandekerckhove
Publikováno v:
Journal of Biological Chemistry. 272:32750-32758
Cofilin is representative for a family of low molecular weight actin filament binding and depolymerizing proteins. Recently the three-dimensional structure of yeast cofilin and of the cofilin homologs destrin and actophorin were resolved, and a strik
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1823c5d86770be8179dd912227ae9fb6
https://doi.org/10.1074/jbc.272.52.32750
https://doi.org/10.1074/jbc.272.52.32750
Publikováno v:
Journal of Protein Chemistry. 16:499-503
Phage display is a technique in which a foreign protein or peptide is presented at the surface of a (filamentous) bacteriophage. This system, developed by Smith [(1985), Science 228, 1315-1317], was originally used to create large libraries of antibo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1272afa407bceae08b49ff351cd382e3
https://doi.org/10.1023/a:1026317612554
https://doi.org/10.1023/a:1026317612554
Autor:
Christophe Ampe, Olivier Debeir, Christine Decaestecker, Daisy Dewitte, Robert J. Eddy, John S. Condeelis, Marleen Van Troys, Shirley Leyman, Laila Ritsma, Jacco van Rheenen, Davy Waterschoot, Joël Vandekerckhove, Mazen Sidani
Publikováno v:
Molecular biology of the cell, 20 (21
Molecular Biology of the Cell, 20(21), 4509-4523. American Society for Cell Biology
Molecular Biology of the Cell, 20(21), 4509-4523. American Society for Cell Biology
Cofilin is a key player in actin dynamics during cell migration. Its activity is regulated by (de)phosphorylation, pH, and binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P(2)]. Here, we here use a human cofilin-1 (D122K) mutant with increas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b46c211a408ea1d1c9673aa052e10a0
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/52937
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/52937
Autor:
Stefaan, Rossenu, Shirley, Leyman, Daisy, Dewitte, Danny, Peelaers, Veronique, Jonckheere, Marleen, Van Troys, Joel, Vandekerckhove, Christophe, Ampe
Publikováno v:
The Journal of biological chemistry. 278(19)
We propose phage display combined with enzyme-linked immunosorbent assay as a tool for the systematic analysis of protein-protein interactions by investigating the binding behavior of variants to a partner protein. Via enzyme-linked immunosorbent ass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::36eeb835c778654202e60c402d8216cc
https://pubmed.ncbi.nlm.nih.gov/12606551
https://pubmed.ncbi.nlm.nih.gov/12606551
Autor:
Daisy Dewitte, Christophe Ampe, Marleen Van Troys, Jean-Luc Verschelde, Marc Goethals, Joël Vandekerckhove
Publikováno v:
Biochemistry. 39(40)
We studied the effect of mutations in an alpha-helical region of actophorin (residues 91-108) on F-actin and PIP(2) binding. As in cofilin, residues in the NH(2)-terminal half of this region are involved in F-actin binding. We show here also that bas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0de8bab6ac5d111a341d9167034bf42e
https://pubmed.ncbi.nlm.nih.gov/11015196
https://pubmed.ncbi.nlm.nih.gov/11015196
We characterized in detail the actin binding site of the small actin-sequestering protein thymosin beta 4 (T beta 4) using chemically synthesized full-length T beta 4 variants. The N-terminal part (residues 1-16) and a hexapeptide motif (residues 17-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91f9d01acffe9fccaa11f824b102267f
https://europepmc.org/articles/PMC449934/
https://europepmc.org/articles/PMC449934/
Publikováno v:
BMC Biochemistry. 3:12
Profilin is a small cytoskeletal protein which interacts with actin, proline-rich proteins and phosphatidylinositol 4,5-bisphosphate (PI(4,5)-P2). Crystallography, NMR and mutagenesis of vertebrate profilins have revealed the amino acid residues that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6587de66f34cfc2becce212ff5b34837
https://doi.org/10.1186/1471-2091-3-12
https://doi.org/10.1186/1471-2091-3-12