Zobrazeno 1 - 10
of 141
pro vyhledávání: '"Daisuke UMENO"'
Publikováno v:
ACS Omega, Vol 9, Iss 14, Pp 16035-16043 (2024)
Externí odkaz:
https://doaj.org/article/50465e993f4a4f50afce95c9e5c66300
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Eukaryotic genetic switches are more complex than prokaryotic ones, complicating their design. Here the authors present a workflow for parallel screening, selection and evolution of yeast genetic switches.
Externí odkaz:
https://doaj.org/article/3522a0a8de284a4c85646d76fb5876fa
Publikováno v:
PLoS ONE, Vol 14, Iss 5, p e0216729 (2019)
Substrate tolerance of bacterial cyclases has been demonstrated in various contexts, but little is known about that of plant cyclases. Here, we tested two plant ε-cyclases to convert C50-lycopene, which we previously established by rounds of directe
Externí odkaz:
https://doaj.org/article/79cc0acd993d4d548acd5bc7a63b8688
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0120243 (2015)
The evolutionary design of genetic switches and circuits requires iterative rounds of positive (ON-) and negative (OFF-) selection. We previously reported a rapid OFF selection system based on the kinase activity of herpes simplex virus thymidine kin
Externí odkaz:
https://doaj.org/article/3c807e43faac40dd8cb0ce1b5c2aca77
Autor:
Masahiro Tominaga, Shigeko Kawai-Noma, Ikuro Kawagishi, Yoshiyuki Sowa, Kyoichi Saito, Daisuke Umeno
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0119818 (2015)
Selection-based recombineering is a flexible and proven technology to precisely modify bacterial genomes at single base resolution. It consists of two steps of homologous recombination followed by selection/counter-selection. However, the shortage of
Externí odkaz:
https://doaj.org/article/b810e59fa99b4476aefb8016511d25ef
Autor:
Maiko Furubayashi, Mayu Ikezumi, Jun Kajiwara, Miki Iwasaki, Akira Fujii, Ling Li, Kyoichi Saito, Daisuke Umeno
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e93317 (2014)
Terpene synthases catalyze the formation of a variety of terpene chemical structures. Systematic mutagenesis studies have been effective in providing insights into the characteristic and complex mechanisms of C-C bond formations and in exploring the
Externí odkaz:
https://doaj.org/article/67df3da6120549faabd3a1e971c4f049
Publikováno v:
Journal of Bioscience and Bioengineering. 133:335-339
Whole-cell sensors for arsenite detection have been developed exclusively based on the natural arsenite (As(III)) sensory protein ArsR for arsenic metabolism. This study reports that the quorum-sensing LuxR/Plux system from Vibrio fischeri, which is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::675c74b0ab2698f129c8bb32894afe81
https://doi.org/10.1016/j.jbiosc.2021.12.013
https://doi.org/10.1016/j.jbiosc.2021.12.013
Publikováno v:
Biochemical and Biophysical Research Communications. 599:75-80
Over 800 known carotenoids are synthesized from phytoene or 4,4'-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3fa6bc8c10486705e88c1c73c0bf4ef
https://doi.org/10.1016/j.bbrc.2022.01.105
https://doi.org/10.1016/j.bbrc.2022.01.105
Autor:
Kanna Sugaya, Satoshi Yasuda, Shingo Sato, Chen Sisi, Taisei Yamamoto, Daisuke Umeno, Tomoaki Matsuura, Tomohiko Hayashi, Satoshi Ogasawara, Masahiro Kinoshita, Takeshi Murata
Publikováno v:
Protein Science. 31
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::59a48f91751df3c2962d98de962c89b1
https://doi.org/10.1002/pro.4404
https://doi.org/10.1002/pro.4404
Autor:
Maiko, Furubayashi, Daisuke, Umeno
Publikováno v:
Methods in enzymology. 671
In recent years, advances in bioengineering and synthetic biology techniques have been used to create carotenoid diversity in the laboratory. In this chapter, we describe the step-by-step method to perform directed evolution of carotenoid biosyntheti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bd759c0c0b008d2d6bb394427bb5d557
https://pubmed.ncbi.nlm.nih.gov/35878986
https://pubmed.ncbi.nlm.nih.gov/35878986