Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Daisuke Kiga"'
Autor:
Jacob Beal, Geoff S Baldwin, Natalie G Farny, Markus Gershater, Traci Haddock-Angelli, Russell Buckley-Taylor, Ari Dwijayanti, Daisuke Kiga, Meagan Lizarazo, John Marken, Kim de Mora, Randy Rettberg, Vishal Sanchania, Vinoo Selvarajah, Abigail Sison, Marko Storch, Christopher T Workman, iGEM Interlab Study Contributors
Publikováno v:
PLoS ONE, Vol 16, Iss 6, p e0252263 (2021)
Reproducibility is a key challenge of synthetic biology, but the foundation of reproducibility is only as solid as the reference materials it is built upon. Here we focus on the reproducibility of fluorescence measurements from bacteria transformed w
Externí odkaz:
https://doaj.org/article/c7ded86e73e9474f93a3c887a1d5cc76
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
Abstract Theories of the origin of the genetic code typically appeal to natural selection and/or mutation of hereditable traits to explain its regularities and error robustness, yet the present translation system presupposes high-fidelity replication
Externí odkaz:
https://doaj.org/article/7282068fede84953aad9112b488260ba
Autor:
Roopa Dharmatti, Hideyuki Miyatake, Avanashiappan Nandakumar, Motoki Ueda, Kenya Kobayashi, Daisuke Kiga, Masayuki Yamamura, Yoshihiro Ito
Publikováno v:
International Journal of Molecular Sciences, Vol 20, Iss 9, p 2152 (2019)
(1) Background: The folate receptor (FR) is a target for cancer treatment and detection. Expression of the FR is restricted in normal cells but overexpressed in many types of tumors. Folate was conjugated with peptides for enhancing binding affinity
Externí odkaz:
https://doaj.org/article/4232d21fe6a84d1f9be07c46bee47f0a
Autor:
Takefumi Moriya, Tomohiro Yamaoka, Yuki Wakayama, Shotaro Ayukawa, Zicong Zhang, Masayuki Yamamura, Shinji Wakao, Daisuke Kiga
Publikováno v:
Life, Vol 9, Iss 1, p 30 (2019)
Reporter genes have contributed to advancements in molecular biology. Binding of an upstream regulatory protein to a downstream reporter promoter allows quantification of the activity of the upstream protein produced from the corresponding gene. In s
Externí odkaz:
https://doaj.org/article/49a30f7d676443ca950330049f6afba6
Autor:
Jacob Beal, Cheryl A Telmer, Alejandro Vignoni, Yadira Boada, Geoff S Baldwin, Liam Hallett, Taeyang Lee, Vinoo Selvarajah, Sonja Billerbeck, Bradley Brown, Guo-nan Cai, Liang Cai, Edward Eisenstein, Daisuke Kiga, David Ross, Nina Alperovich, Noah Sprent, Jaclyn Thompson, Eric M Young, Drew Endy, Traci Haddock-Angelli
Publikováno v:
Synthetic Biology, 7(1), 1-9
Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously established a ro
Autor:
Naoko Senda, Toshihiko Enomoto, Kenta Kihara, Naoki Yamashiro, Naosato Takagi, Daisuke Kiga, Hirokazu Nishida
Publikováno v:
Synthetic biology (Oxford, England). 7(1)
New materials with a low environmental load are expected to be generated through synthetic biology. To widely utilize this technology, it is important to create cells with designed biological functions and to control the expression of multiple enzyme
Autor:
Takehiro Suzuki, Michinori Kohara, Takashi Imada, Naoshi Dohmae, Yo Ichi Tagawa, Koji Moriya, Mitsuhiro Hitotsuyanagi, Naoto Inukai, Masahiko Uchiyama, Daisuke Kiga, Shotaro Ayukawa, Akiko Masuda, Masayuki Yamamura
Publikováno v:
ACS Synthetic Biology. 7:2537-2546
Although conjugation with polyethylene glycol (PEGylation) improves the pharmacokinetics of therapeutic proteins, it drastically decreases their bioactivity. Site-specific PEGylation counters the reduction in bioactivity, but developing PEGylated pro
Autor:
John P. Marken, Russell Buckley-Taylor, Randy Rettberg, Natalie G. Farny, Marko Storch, Vinoo Selvarajah, Daisuke Kiga, iGEM Interlab Study Contributors, Vishal Sanchania, Meagan Lizarazo, Traci Haddock-Angelli, Christopher T. Workman, Ari Dwijayanti, Abigail Sison, Jacob Beal, Geoff S. Baldwin, Kim de Mora, Markus Gershater
Publikováno v:
PLoS ONE, Vol 16, Iss 6, p e0252263 (2021)
PLoS ONE
PLoS ONE, 16(6)
Interlab Study Contributors iGEM, Beal, J, Baldwin, G S, Farny, N G, Gershater, M, Haddock-Angelli, T, Buckley-Taylor, R, Dwijayanti, A, Kiga, D, Lizarazo, M, Marken, J, de Mora, K, Rettberg, R, Sanchania, V, Selvarajah, V, Sison, A, Storch, M & Workman, C T 2021, ' Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs ', PLOS ONE, vol. 16, no. 6, e0252263 . https://doi.org/10.1371/journal.pone.0252263
PLoS ONE
PLoS ONE, 16(6)
Interlab Study Contributors iGEM, Beal, J, Baldwin, G S, Farny, N G, Gershater, M, Haddock-Angelli, T, Buckley-Taylor, R, Dwijayanti, A, Kiga, D, Lizarazo, M, Marken, J, de Mora, K, Rettberg, R, Sanchania, V, Selvarajah, V, Sison, A, Storch, M & Workman, C T 2021, ' Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs ', PLOS ONE, vol. 16, no. 6, e0252263 . https://doi.org/10.1371/journal.pone.0252263
Reproducibility is a key challenge of synthetic biology, but the foundation of reproducibility is only as solid as the reference materials it is built upon. Here we focus on the reproducibility of fluorescence measurements from bacteria transformed w
Publikováno v:
BMC Systems Biology. 12/21/2017, Vol. 11, p151-162. 12p.
Autor:
Vishal Sanchania, Daisuke Kiga, John P. Marken, Abigail Sison, Jacob Beal, Russell Buckley-Taylor, Markus Gershater, Natalie G. Farny, Geoff S. Baldwin, Vinoo Selvarajah, Traci Haddock-Angelli, iGEM Interlab Study Contributors, Christopher T. Workman
Publikováno v:
Communications Biology
Communications biology, vol 3, iss 1
Communications Biology, Vol 3, Iss 1, Pp 1-1 (2020)
Communications biology, vol 3, iss 1
Communications Biology, Vol 3, Iss 1, Pp 1-1 (2020)
Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an