Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Daisuke Kajihara"'
Autor:
Hiroki Tanemura, Kenji Masuda, Takeshi Okumura, Eri Takagi, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka, Ryo Ushioda
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Abstract Chinese hamster ovary (CHO) cells are widely used for manufacturing antibody drugs. We attempted to clone a novel high-expression promoter for producing monoclonal antibodies (mAbs) based on transcriptome analysis to enhance the transcriptio
Externí odkaz:
https://doaj.org/article/fcda47d8d6944ae2a31afe14caac6a4e
Publikováno v:
Nature Methods. 3:923-929
We designed and synthesized new, fluorescent, non-natural amino acids that emit fluorescence of wavelengths longer than 500 nm and are accepted by an Escherichia coli cell-free translation system. We synthesized p-aminophenylalanine derivatives linke
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24f8bbb74fb3f0d11b37826e8255fa11
https://doi.org/10.1038/nmeth945
https://doi.org/10.1038/nmeth945
Publikováno v:
Journal of the American Chemical Society. 121:34-40
Efficiencies of the incorporation of various nonnatural amino acids carrying aromatic side groups into streptavidin were examined. The aromatic amino acids were linked to a mixed dinucleotide, pdCpA, and the resulting aminoacyl pdCpAs were coupled wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d0447696161f3427660a5ada1bad3c9f
https://doi.org/10.1021/ja9813109
https://doi.org/10.1021/ja9813109
Autor:
Shinya Tashiro, Daisuke Kajihara, Jose M. M. Caaveiro, Tomoya Mitani, Akihiro Kobe, Masato Kikkawa, Kouhei Tsumoto
Publikováno v:
Journal of medicinal chemistry. 56(5)
Fragment-based drug discovery (FBDD) has enjoyed increasing popularity in recent years. We introduce SITE (single-injection thermal extinction), a novel thermodynamic methodology that selects high-quality hits early in FBDD. SITE is a fast calorimetr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::965b45b627448e973bb0ebaa152c7555
https://pubmed.ncbi.nlm.nih.gov/23419007
https://pubmed.ncbi.nlm.nih.gov/23419007
Publikováno v:
Chemphyschem : a European journal of chemical physics and physical chemistry. 7(8)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02338858dd875fbc201d00b1aa72ed26
https://pubmed.ncbi.nlm.nih.gov/16832804
https://pubmed.ncbi.nlm.nih.gov/16832804
Publikováno v:
Protein engineering, designselection : PEDS. 18(6)
In order to alter the fluorescence properties of green fluorescent protein (GFP), aromatic non-natural amino acids were introduced into the Tyr66 position of GFP in a cell-free translation system using a four-base codon method. Two non-natural mutant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::442dde9ea4edfa0785c59f425a7344ed
https://pubmed.ncbi.nlm.nih.gov/15928004
https://pubmed.ncbi.nlm.nih.gov/15928004
Publikováno v:
PEDS: Protein Engineering, Design & Selection; Jun2005, Vol. 18 Issue 6, p273-278, 6p
Autor:
Noriko Katori, Maiko Tsutsui, Nana Kawasaki, Hideshi Hattori, Norikata Hashii, H Matsuda, Shinya Takuma, Masahiro Iwakura, Daisuke Kajihara, Takeshi Omasa, Tetsuya Isono, Akiko Ishii-Watabe, Nobuo Kasahara, Takehiro Okumura, Nakagawa Taishiro, Iyo Terashima, Murakami Sei, Masayoshi Tsukahara, Hirotoshi Awatsu, Takahiro Yano, Akihiko Hirose, Tomomi Inoue, Susumu Arai, Akira Eiza, Yoshiaki Hara
Publikováno v:
AAPS PharmSciTech
Biologics manufacturing technology has made great progress in the last decade. One of the most promising new technologies is the single-use system, which has improved the efficiency of biologics manufacturing processes. To ensure safety of biologics
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9991fbff84a734f30c27cf83d09aad1e
Publikováno v:
ChemPhysChem; Aug2006, Vol. 7 Issue 8, p1671-1673, 3p