Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Daisuke Fujinami"'
Autor:
Yui Kawamura, Chiharu Ishida, Ryo Miyata, Azusa Miyata, Seiichiro Hayashi, Daisuke Fujinami, Sohei Ito, Shogo Nakano
Publikováno v:
Communications Chemistry, Vol 6, Iss 1, Pp 1-10 (2023)
Abstract Production of D-amino acids (D-AAs) on a large-scale enables to provide precursors of peptide therapeutics. In this study, we designed a novel L-amino acid oxidase, HTAncLAAO2, by ancestral sequence reconstruction, exhibiting high thermostab
Externí odkaz:
https://doaj.org/article/df1371eaef6541c18fdebc8e1b4aa00f
Publikováno v:
Advanced Science, Vol 11, Iss 6, Pp n/a-n/a (2024)
Abstract Post‐translational prenylations, found in eukaryotic primary metabolites and bacterial secondary metabolites, play crucial roles in biomolecular interactions. Employing genome mining methods combined with AlphaFold2‐based predictions of
Externí odkaz:
https://doaj.org/article/5b9cc45e381b4b2e9f846e52cf80e5ec
Publikováno v:
Biophysics and Physicobiology, Vol 20 (2023)
The consistency principle represents a physicochemical condition requisite for ideal protein folding. It assumes that any pair of amino acid residues in partially folded structures has an attractive short-range interaction only if the two residues ar
Externí odkaz:
https://doaj.org/article/74698be165494278a8149f96d31fa103
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-11 (2022)
Abstract Multiprobe measurements, such as NMR and hydrogen exchange studies, can provide the equilibrium constant, K, and rate constants for forward and backward processes, k and k′, of the two-state structural changes of a polypeptide on a per-res
Externí odkaz:
https://doaj.org/article/0a645bb0dad946faaf7fb01e5af09037
Autor:
Naomi Inoue, Takeshi Terabayashi, Yuri Takiguchi-Kawashima, Daisuke Fujinami, Shigeru Matsuoka, Masanori Kawano, Kazuhiro Tanaka, Hiroshi Tsumura, Toshimasa Ishizaki, Hisashi Narahara, Daisuke Kohda, Yoshihiro Nishida, Katsuhiro Hanada
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
Abstract DNA replication inhibitors are utilized extensively in studies of molecular biology and as chemotherapy agents in clinical settings. The inhibition of DNA replication often triggers double-stranded DNA breaks (DSBs) at stalled DNA replicatio
Externí odkaz:
https://doaj.org/article/e25dffc15303468893e8fa92c6174e41
Autor:
Yui Kawamura, Chiharu Ishida, Ryo Miyata, Azusa Miyata, Seiichiro Hayashi, Daisuke Fujinami, Sohei Ito, Shogo Nakano
Establishing a reaction system to produce D-amino acids (D-AAs) on a large-scale has been required for now. In this study, we designed a novel L-amino acid oxidase, HTAncLAAO2, by ancestral sequence reconstruction, exhibiting high thermostability and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4b2170ca865b8cd3f727bd102694902f
https://doi.org/10.21203/rs.3.rs-2969058/v1
https://doi.org/10.21203/rs.3.rs-2969058/v1
Publikováno v:
The Journal of Physical Chemistry Letters. 12:10551-10557
The characterization of the transition state is a central issue in biophysical studies of protein folding. NMR is a multiprobe measurement technique that provides residue-specific information. Here, we used exchange spectroscopy to characterize the t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1528568e988a83ff2fae8d27071223fb
https://doi.org/10.1021/acs.jpclett.1c02387
https://doi.org/10.1021/acs.jpclett.1c02387
The consistency principle represents a physicochemical condition requisite for ideal protein folding. The consistency principle assumes that any pair of amino acid residues in partially folded structures has an attractive short-range interactiononly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b0fb396c4e530b93eef3334bfc894c1f
https://doi.org/10.1101/2022.10.24.513469
https://doi.org/10.1101/2022.10.24.513469
Autor:
Hirotaka Ariyama, Hajime Motomura, Toshio Ando, Yuki Kawasaki, Daisuke Kohda, Daisuke Noshiro, Daisuke Fujinami
Publikováno v:
Journal of Molecular Biology. 432:5951-5965
Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains. Using high-speed atomic force microscopy (AFM), we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40b96608db3453d134fbca7dab4fb983
https://doi.org/10.1016/j.jmb.2020.09.017
https://doi.org/10.1016/j.jmb.2020.09.017
Multiprobe measurements, such as NMR and hydrogen exchange study, can provide the equilibrium constant K and kinetic rate constant k of the structural changes of a polypeptide on a per-residue basis. We previously found a linear relationship between
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5ac2d06f7f82fb6ac4399099e798fef2
https://doi.org/10.1101/2022.01.07.475459
https://doi.org/10.1101/2022.01.07.475459