Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Daija Bobe"'
Autor:
Daniel Asarnow, Vada A. Becker, Daija Bobe, Charlie Dubbledam, Jake D. Johnston, Mykhailo Kopylov, Nathalie R. Lavoie, Qiuye Li, Jacob M. Mattingly, Joshua H. Mendez, Mohammadreza Paraan, Jack Turner, Viraj Upadhye, Richard M. Walsh, Meghna Gupta, Edward T. Eng
Publikováno v:
Frontiers in Molecular Biosciences, Vol 10 (2024)
With the increasing spread of infectious diseases worldwide, there is an urgent need for novel strategies to combat them. Cryogenic sample electron microscopy (cryo-EM) techniques, particularly electron tomography (cryo-ET), have revolutionized the f
Externí odkaz:
https://doaj.org/article/7ec122d9736b4a21a3b17168d50d7ab1
Autor:
Kotaro Kelley, Ashleigh M. Raczkowski, Oleg Klykov, Pattana Jaroenlak, Daija Bobe, Mykhailo Kopylov, Edward T. Eng, Gira Bhabha, Clinton S. Potter, Bridget Carragher, Alex J. Noble
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Here the authors describe the Waffle Method, aimed at increasing the throughput of and solves several challenges present in cryo-FIB/SEM sample preparation for cryo-ET analysis — the highest-resolution method for obtaining 3D views of native biolog
Externí odkaz:
https://doaj.org/article/e079e893c227458c8f9d1347d6358ed8
Autor:
Oleg Klykov, Daija Bobe, Mohammadreza Paraan, Jake Johnston, Clinton Potter, Bridget Carragher, Mykhailo Kopylov, Alex Noble
Publikováno v:
Bio-Protocol, Vol 12, Iss 21 (2022)
Cryo-focused ion beam (FIB) milling of vitrified specimens is emerging as a powerful method for in situ specimen preparation. It allows for the preservation of native and near-native conditions in cells, and can reveal the molecular structure of prot
Externí odkaz:
https://doaj.org/article/82aefb10e3d8473a81eecfaa299accd1
Autor:
Laura Y. Kim, William J. Rice, Edward T. Eng, Mykhailo Kopylov, Anchi Cheng, Ashleigh M. Raczkowski, Kelsey D. Jordan, Daija Bobe, Clinton S. Potter, Bridget Carragher
Publikováno v:
Frontiers in Molecular Biosciences, Vol 5 (2018)
Cryo electron microscopy facilities running multiple instruments and serving users with varying skill levels need a robust and reliable method for benchmarking both the hardware and software components of their single particle analysis workflow. The
Externí odkaz:
https://doaj.org/article/6aa7a9a89779493995198451bdcb91a6
Autor:
Kotaro Kelley, Mykhailo Kopylov, Kookjoo Kim, Edward T. Eng, Oliver B. Clarke, Daija Bobe, Peter Arvan
Publikováno v:
Acta Crystallographica. Section D, Structural Biology
Thyroglobulin is the precursor protein that serves as a substrate for thyroid hormone synthesis. Here, a three-dimensional cryoEM reconstruction of bovine thyroglobulin at an overall resolution of 2.6 Å is presented. The structure facilitates the de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::329c27c4f52a22309d18fb38938d3022
https://doi.org/10.1107/s2059798321010056
https://doi.org/10.1107/s2059798321010056
Autor:
Daija Bobe, Gira Bhabha, Alex J. Noble, Clinton S. Potter, Mykhailo Kopylov, Oleg Klykov, Kotaro Kelley, Bridget Carragher, Edward T. Eng, Pattana Jaroenlak, Ashleigh M. Raczkowski
Publikováno v:
Nature Communications. 13
Cryo-FIB/SEM combined with cryo-ET has emerged from within the field of cryo-EM as the method for obtaining the highest resolution structural information of complex biological samples in-situ in native and non-native environments. However, challenges
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed5fc20041bc76cef74377c7f3c40ab1
https://doi.org/10.1038/s41467-022-29501-3
https://doi.org/10.1038/s41467-022-29501-3
Publikováno v:
Microscopy and Microanalysis. 28:1248-1248
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b2b4c2d62f544839e16d0ff26cb7d44c
https://doi.org/10.1017/s1431927622005177
https://doi.org/10.1017/s1431927622005177
Autor:
Elina Kopylov, Bridget Carragher, Misha Kopylov, Daija Bobe, Clint Potter, Charlie Dubbeldam, Alex J. Noble, Ashleigh M. Raczkowski
Publikováno v:
Microscopy and Microanalysis. 27:2298-2299
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d3b183c45c08feac2dc844fc073cdbbe
https://doi.org/10.1017/s1431927621008266
https://doi.org/10.1017/s1431927621008266
Autor:
Mykhailo Kopylov, Crina M. Nimigean, Clint Potter, Hui Wei, Nattakan Sukomon, Ruth M. Saecker, Venkata P. Dandey, William C Budell, Jenny E. Hinshaw, Brandon Malone, Chen Fan, Kashyap Maruthi, Daija Bobe, Peter A. Kahn, Nidhi Kundu, James Chen, Bridget Carragher, Edward T. Eng, Seth A. Darst
We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a42b90b3cb991cdba1288a99831e5a82
https://doi.org/10.1101/2020.04.20.050518
https://doi.org/10.1101/2020.04.20.050518
Autor:
Chen Fan, Venkata P. Dandey, Kashyap Maruthi, Nidhi Kundu, Peter A. Kahn, Clinton S. Potter, Seth A. Darst, Nattakan Sukomon, Crina M. Nimigean, Mykhailo Kopylov, Jenny E. Hinshaw, Ruth M. Saecker, Hui Wei, Daija Bobe, James Chen, Bridget Carragher, Edward T. Eng, Brandon Malone, William C Budell
Publikováno v:
Nature methods
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e8d3ffb68d72cbae9ca8ab2a5fda6ce
https://pubmed.ncbi.nlm.nih.gov/32778833
https://pubmed.ncbi.nlm.nih.gov/32778833