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pro vyhledávání: '"Dagmar Schmiedel"'
Publikováno v:
Molecular Microbiology. 23:1053-1062
Xylose uptake in Bacillus megaterium depends on expression of a putative H+/xylose symporter encoded by xylT, the last gene in the xyl operon. Insertional inactivation of xylT leads to an apparent uptake deficiency determined with whole cells and sev
Autor:
Dagmar Schmiedel, Wolfgang Hillen
Publikováno v:
FEMS Microbiology Letters. 135:175-178
Bacillus subtilis 168 is unable to effectively utilize xylose as sole carbon source. We demonstrate here that this strain cannot actively transport xylose into the cell. After leaving B. subtilis 168 for a few days on minimal plates with xylose as so
Publikováno v:
FEMS Microbiology Letters. 109:81-84
We have selected a Bacillus subtilis 168-borne xylR Ser to Leu mutation at position 41 of the encoded amino acid sequence showing a constitutive expression phenotype for the xyl operon. When cloned on a multi-copy plasmid in a B. megaterium strain ha
Autor:
Wolfgang Hillen, Dagmar Schmiedel
Publikováno v:
Moleculargeneral genetics : MGG. 250(3)
Bacillus megaterium shows diauxic growth in minimal medium containing glucose and xylose. We have examined the influence of three elements that regulate xyl operon expression on diauxic growth and expression of a xylA-lacZ fusion. xylA is 13-fold rep
Publikováno v:
Molecular microbiology. 16(5)
A mutant of Bacillus megaterium relieved from catabolite repression has been used to clone ccpA from B. megaterium by complementation. ccpA is the first gene of a presumed operon, in which it is followed by the motA homologue ORF1 and the motB homolo
Autor:
Wolfgang Hillen, Dagmar Schmiedel
Publikováno v:
MGG Molecular & General Genetics. 250:259