Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Dae Nyun Kim"'
Publikováno v:
Reactive and Functional Polymers. 69:293-299
In this study, hydrogel microparticles containing enzyme-linked magnetic nanoparticles (MNPs) were prepared. Peroxidase (POD), a model enzyme, was covalently immobilized on the surface of MNPs using 3-aminopropyltriethoxysilane (APTES), and the resul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c120b5c9f27f2ecb32a0017fde22e4f
https://doi.org/10.1016/j.reactfunctpolym.2009.02.001
https://doi.org/10.1016/j.reactfunctpolym.2009.02.001
Publikováno v:
Sensors and Actuators B: Chemical. 137:305-312
In this study, we developed a novel microfluidic device for enzyme-based assays that incorporates heterogeneous reaction and microarray-based detection systems. The microfluidic device had two serial chambers connected through microchannels; one for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1278fdd4ac0efc739235ede3d636d26e
https://doi.org/10.1016/j.snb.2008.12.042
https://doi.org/10.1016/j.snb.2008.12.042
Publikováno v:
Journal of Chemical Technology & Biotechnology. 84:279-284
BACKGROUND: Conventional protein microarrays prepared on hard, dry substrates, such as glass and silicone, have several limitations, as proteins may easily denature and lose their structure. To overcome such problems, the fabrication of wet protein m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68afe3e96debcd95ba793a5e01af8144
https://doi.org/10.1002/jctb.2036
https://doi.org/10.1002/jctb.2036
Publikováno v:
Journal of Industrial and Engineering Chemistry. 15:124-128
Cell and protein-repellent poly(ethylene glycol) (PEG) hydrogels surfaces were modified to covalently attach cell adhesion proteins and subsequently promote cell adhesion. Collagen was chosen as a cell adhesion protein and covalently immobilized to t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a3a6c8174543ba30a7d5b115c867a323
https://doi.org/10.1016/j.jiec.2008.08.001
https://doi.org/10.1016/j.jiec.2008.08.001
Publikováno v:
Polymers for Advanced Technologies. 19:852-858
Poly(ethylene glycol)(PEG)-based interpenetrating polymeric network (IPN) hydrogels were prepared for the application of enzyme immobilization. Poly(acrylamide)(PAAm) was chosen as the other network of IPN hydrogel and different concentration of PAAm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0ef14e7d874fb3d9719229f8a2633a29
https://doi.org/10.1002/pat.1047
https://doi.org/10.1002/pat.1047
Publikováno v:
Sensors and Actuators B: Chemical. 129:841-849
This paper presents a simple method to create protein micropattern on the poly(ethylene glycol) (PEG) hydrogels through the surface graft polymerization and photolithography. The modification of the protein-repellent PEG hydrogel surface was achieved
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4e3f2a59cc6ffc44bf4b7eb4942f2117
https://doi.org/10.1016/j.snb.2007.09.085
https://doi.org/10.1016/j.snb.2007.09.085
Publikováno v:
Journal of Chemical Technology & Biotechnology. 83:252-259
BACKGROUND: The main problem when immobilizing enzyme inside hydrogel is the activity loss of the trapped enzymes due to the limitation on diffusion. In this study, macroporous poly(ethylene glycol) (PEG) hydrogel membrane was fabricated using photol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a7d6cc0285c704827bee401901e3e8d4
https://doi.org/10.1002/jctb.1800
https://doi.org/10.1002/jctb.1800
Publikováno v:
FEMS immunology and medical microbiology. 66(3)
To better understand the role of host cell-derived molecules on Enterohemorrhagic Escherichia coli (EHEC) infection, we studied EHEC virulence gene expression when exposed to cell-free spent (conditioned) medium (CM) from HCT-8 intestinal epithelial
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a3ec2cba3a88a65dee6b666b63dab2a
https://pubmed.ncbi.nlm.nih.gov/23163871
https://pubmed.ncbi.nlm.nih.gov/23163871
Publikováno v:
Acta biomaterialia. 7(3)
In this paper, we describe a simple method for fabricating micropatterned nanoporous substrates that are capable of controlling the spatial positioning of mammalian cells. Micropatterned substrates were prepared by fabricating poly(ethylene glycol) (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbfa1c323cb65d4128eb016a00f508ce
https://pubmed.ncbi.nlm.nih.gov/21056702
https://pubmed.ncbi.nlm.nih.gov/21056702
Autor:
Sangphial Park, Bumsang Kim, Yeol Lim Lee, Eunji Jang, Sabemi Park, Won Gun Koh, Dae Nyun Kim
Publikováno v:
Polymers for Advanced Technologies.
In this study, we immobilized enzymes by combining covalent surface immobilization and hydrogel entrapment. A model enzyme, glucose oxidase (GOX), was first covalently immobilized on the surface of silica nanoparticles (SNPs) via 3-aminopropyltrietho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e0efcb2e17b70da1b3ccb4259ab5b95e
https://doi.org/10.1002/pat.1455
https://doi.org/10.1002/pat.1455