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pro vyhledávání: '"Da Qun Ni"'
Publikováno v:
Protein Expression and Purification. 80:157-168
Membrane proteins compose more than 30% of all proteins in the living cell. However, many membrane proteins have low abundance in the cell and cannot be isolated from natural sources in concentrations suitable for structure analysis. The overexpressi
Publikováno v:
Protein science : a publication of the Protein Society. 9(9)
The binding of the methylanthraniloyl derivatives of ATP (mant-ATP), ADP (mant-ADP), 2′deoxyATP (mant-2′deoxyATP), and 3′deoxyATP (mant-3′deoxyATP) to the catalytic subunit of protein kinase A was studied to gain insights into the mechanism o
Publikováno v:
Biophysical Journal. 96(3):336a-337a
Here is a novel procedure for making a membrane protein sample, Lipoprotein Signal Peptidase (LspA) for structural studies by solid state NMR. LspA is expressed in E.Coli, purified and refolded with detergent on a Ni-NTA affinitive column and uniform