Zobrazeno 1 - 10
of 300
pro vyhledávání: '"DONALD J. WINZOR"'
Autor:
Donald J. Winzor
Publikováno v:
Quantitative Analysis of Biospecific Interactions ISBN: 9781315824994
As a sequel to the successful exploitation of affinity chromatography for the purification of solutes, quantitative affinity chromatography was developed to take advantage of the same chromatographic matrix for determination of the binding constants
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7aabfe1bf760e495673c7859624c1b80
https://doi.org/10.4324/9781315824994-3
https://doi.org/10.4324/9781315824994-3
Autor:
Donald J. Winzor
Publikováno v:
Quantitative Analysis of Biospecific Interactions ISBN: 9781315824994
Chapters 3 and 5 on the measurement of binding constants by frontal affinity chromatography emphasize that elution volumes are merely quantitative indices of the equilibrium position of solute partition between soluble and adsorbed (matrix-bound) sta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01b0c4514d18300eea0bf13d5d65ebf3
https://doi.org/10.4324/9781315824994-4
https://doi.org/10.4324/9781315824994-4
Publikováno v:
European Biophysics Journal. 48:781-787
This investigation examines the validity of employing single-solute theory to interpret SAXS measurements on buffered protein solutions—the current practice despite the necessity to regard the buffer components as additional non-scattering solutes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::430397beb4946a4eb4850372c2952148
https://doi.org/10.1007/s00249-019-01404-0
https://doi.org/10.1007/s00249-019-01404-0
Publikováno v:
European Biophysics Journal
This investigation of the temperature dependence of DppA interactions with a subset of three dipeptides (AA. AF and FA) by isothermal titration calorimetry has revealed the negative heat capacity ($$\Delta {C}_{p}^{o}$$ΔCpo) that is a characteristic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dfc5af769c661e91a244f8c8d353cfc
https://pubmed.ncbi.nlm.nih.gov/34611772
https://pubmed.ncbi.nlm.nih.gov/34611772
Publikováno v:
Biophysical Reviews
This retrospective investigation has established that the early theoretical attempts to directly incorporate the consequences of radial dilution into expressions for variation of the sedimentation coefficient as a function of the loading concentratio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3db56dfd3e24f2e2d9bf48f6d5772c6d
Autor:
Stephen E. Harding, Kevin McEleney, Jörg Stetefeld, Markus Meier, Tabot M. D. Besong, Trushar R. Patel, Donald J. Winzor
Publikováno v:
European Biophysics Journal. 47:751-759
The major challenges in biophysical characterization of human protein-carbohydrate interactions are obtaining monodispersed preparations of human proteins that are often post-translationally modified and lack of detection of carbohydrates by traditio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3fdf84d4cecd1a1207e80b222cd1720
https://doi.org/10.1007/s00249-018-1291-5
https://doi.org/10.1007/s00249-018-1291-5
Publikováno v:
European Biophysics Journal. 47:291-295
The accuracy with which the concentration dependence of the sedimentation coefficient, s = s 0(1 − kc), can be quantified for globular proteins by commonly used procedures has been examined by subjecting simulated sedimentation velocity distributio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::487c3c1f51aeea5699a254827e087440
https://doi.org/10.1007/s00249-017-1259-x
https://doi.org/10.1007/s00249-017-1259-x
Publikováno v:
Biochimie. 135:6-14
Sedimentation equilibrium and size-exclusion chromatography experiments on Mycobacterium tuberculosis hypoxanthine-guanine phosphoribosyltransferase (MtHGPRT) have established the existence of this enzyme as a reversibly associating mixture of dimeri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c885874ad3eb7769d48c0b34cbd042b1
https://doi.org/10.1016/j.biochi.2016.12.020
https://doi.org/10.1016/j.biochi.2016.12.020
Publikováno v:
Analytical biochemistry. 584
The feasibility of employing molecular crowding cosolutes to facilitate the detection of protein self-association by zonal size exclusion chromatography is investigated. Theoretical considerations have established that although the cosolute-induced d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89e932928e52ef12fffecfa29ddb571c
https://pubmed.ncbi.nlm.nih.gov/31408631
https://pubmed.ncbi.nlm.nih.gov/31408631
Autor:
Donald J. Winzor, Peter R. Wills
Publikováno v:
Biophysical chemistry. 251
This investigation amends the analysis of isopiestic measurements of solvent thermodynamic activity by taking into account the fact that the solvent activity, traditionally expressed in mole-fraction terms, is a molal parameter because of the constra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f766edcb1db9db8fc58b02856881c133
https://pubmed.ncbi.nlm.nih.gov/31128561
https://pubmed.ncbi.nlm.nih.gov/31128561