Zobrazeno 1 - 10
of 183
pro vyhledávání: '"DNA vector"'
Autor:
Juan Angulo, Christopher P. Astin, Olivia Bauer, Kelan J. Blash, Natalee M. Bowen, Nneoma J. Chukwudinma, Austin S. DiNofrio, Donald O. Faletti, Alexa M. Ghulam, Chloe M. Gusinde-Duffy, Kamaria J. Horace, Andrew M. Ingram, Kylie E. Isaack, Geon Jeong, Randolph J. Kiser, Jason S. Kobylanski, Madeline R. Long, Grace A. Manning, Julie M. Morales, Kevin H. Nguyen, Robin T. Pham, Monthip H. Phillips, Tanner W. Reel, Jenny E. Seo, Hiep D. Vo, Alexander M. Wukoson, Kathryn A. Yeary, Grace Y. Zheng, Wolfgang Lukowitz
Publikováno v:
Frontiers in Genome Editing, Vol 5 (2023)
Genome editing in plants typically relies on T-DNA plasmids that are mobilized by Agrobacterium-mediated transformation to deliver the CRISPR/Cas machinery. Here, we introduce a series of CRISPR/Cas9 T-DNA vectors for minimal settings, such as teachi
Externí odkaz:
https://doaj.org/article/31f8f2f41e594d5e9d3f195f4e86f65e
Autor:
Tatjana Chan, Hiu Man Grisch-Chan, Philipp Schmierer, Ulrike Subotic, Nicole Rimann, Tanja Scherer, Udo Hetzel, Matthias Bozza, Richard Harbottle, James A. Williams, Barbara Steblaj, Simone K. Ringer, Johannes Häberle, Xaver Sidler, Beat Thöny
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 24, Iss , Pp 268-279 (2022)
Hepatic gene therapy by delivering non-integrating therapeutic vectors in newborns remains challenging due to the risk of dilution and loss of efficacy in the growing liver. Previously we reported on hepatocyte transfection in piglets by intraportal
Externí odkaz:
https://doaj.org/article/9af6b8771dc44a3caf3414a328b73cff
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 81-93 (2021)
Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be enhanced through genetic modification. However, gene delivery, particularly through nonviral routes, is inefficient. To address the shortcomings of nonv
Externí odkaz:
https://doaj.org/article/d9f806a20b4e4165b55a9a921d4e5c78
Publikováno v:
Plant Methods, Vol 16, Iss 1, Pp 1-17 (2020)
Abstract Background Generation of marker-free transgenic plants is very important to the regulatory permission and commercial release of transgenic crops. Co-transformation methods that enable the removal of selectable marker genes have been extensiv
Externí odkaz:
https://doaj.org/article/1e3c60de24b44062a285918cfeaf14eb
Autor:
David C. Bishop, Lisa Caproni, Kavitha Gowrishankar, Michal Legiewicz, Kinga Karbowniczek, John Tite, David J. Gottlieb, Kenneth P. Micklethwaite
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 17, Iss , Pp 359-368 (2020)
CD19-specific chimeric antigen receptor (CAR19) T cells, generated using viral vectors, are an efficacious but costly treatment for B cell malignancies. The nonviral piggyBac transposon system provides a simple and inexpensive alternative for CAR19 T
Externí odkaz:
https://doaj.org/article/83da7d10b4244e449b536afbd1317924
Autor:
Matthias Bozza, Edward W. Green, Elisa Espinet, Alice De Roia, Corinna Klein, Vanessa Vogel, Rienk Offringa, James A. Williams, Martin Sprick, Richard P. Harbottle
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 17, Iss , Pp 957-968 (2020)
We describe herein non-integrating minimally sized nano-S/MAR DNA vectors, which can be used to genetically modify dividing cells in place of integrating vectors. They represent a unique genetic tool, which avoids vector-mediated damage. Previous wor
Externí odkaz:
https://doaj.org/article/c209bacd632e4cabbf2898e259b2e84f
Akademický článek
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Publikováno v:
Frontiers in Chemical Engineering, Vol 2 (2021)
Minicircles are non-viral delivery vectors with promising features for biopharmaceutical applications. These vectors are plasmid-derived circular DNA molecules that are obtained in vivo in Escherichia coli by the intramolecular recombination of a par
Externí odkaz:
https://doaj.org/article/af8549c2e62d4403a8fd8084165835c2
Autor:
Ek Han Tan, Benny Ordoñez, Tejas Thondehaalmath, Danelle K. Seymour, Julin N. Maloof, Ravi Maruthachalam, Luca Comai
Publikováno v:
Chromosoma, vol 132, iss 2
Minichromosomes are small, sometimes circular, rearranged chromosomes consisting of one centromere and short chromosomal arms, formed by treatments that break DNA, including plant transformation. Minichromosomes have the potential to serve as vectors
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b3f313ada99322c805e55b45843840d
https://doi.org/10.1007/s00412-023-00788-5
https://doi.org/10.1007/s00412-023-00788-5
Autor:
Alicia Roig-Merino, Manuela Urban, Matthias Bozza, Julia D. Peterson, Louise Bullen, Marleen Büchler-Schäff, Sina Stäble, Franciscus van der Hoeven, Karin Müller-Decker, Tristan R. McKay, Michael D. Milsom, Richard P. Harbottle
Publikováno v:
Stem Cell Reports
Summary The genetic modification of stem cells (SCs) is typically achieved using integrating vectors, whose potential integrative genotoxicity and propensity for epigenetic silencing during differentiation limit their application. The genetic modific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf1c6159815ed2ed5c80b04325b639d9
https://doi.org/10.1016/j.stemcr.2021.11.011
https://doi.org/10.1016/j.stemcr.2021.11.011