Zobrazeno 1 - 10
of 1 208
pro vyhledávání: '"DNA assembly"'
Autor:
Petra Paclikova, Jakub Harnos
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-8 (2024)
Abstract This study introduces a novel cost-effective technique for cloning of linear DNA plasmid inserts, aiming to address the associated expenses linked with popular in vitro DNA assembly methods. Specifically, we introduce ECOLI (Efficient Clonin
Externí odkaz:
https://doaj.org/article/5818acc443d84b378466330b68425ab5
Autor:
Yu Yang, Hao Lu, Dan Fang, Yuyuan Zhang, Yuteng Tang, Songsong Zhao, Jun Yan, Xiaojie Qin, Jianlei Shen, Fan Yang
Publikováno v:
Aggregate, Vol 5, Iss 6, Pp n/a-n/a (2024)
Abstract Solid bubbles have expanded the SERS assay toolbox, but their detection performance in biofluids is still hampered by the irrational design of the plasmonic sensing interface. A plasmonic bubble aggregate‐driven DNA‐encoded SERS assay is
Externí odkaz:
https://doaj.org/article/ad4f7af39a7d4d7b9d2c2761097de25e
Publikováno v:
Advanced Science, Vol 11, Iss 46, Pp n/a-n/a (2024)
Abstract Differential RNA expression is becoming increasingly valuable in evaluating tumor heterogeneity for a better understanding of malignant tumors and guiding personalized therapy. However, traditional techniques for analyzing cellular RNA are m
Externí odkaz:
https://doaj.org/article/c937a93487a34812abd0b73e6c902fe8
Publikováno v:
Synthetic and Systems Biotechnology, Vol 9, Iss 1, Pp 69-77 (2024)
Streptomyces has enormous potential to produce novel natural products (NPs) as it harbors a huge reservoir of uncharacterized and silent natural product biosynthetic gene clusters (BGCs). However, the lack of efficient gene cluster engineering strate
Externí odkaz:
https://doaj.org/article/b5bf941dbad544398d70c700a365b533
Publikováno v:
Microorganisms, Vol 12, Iss 7, p 1462 (2024)
The ability to clone large DNA fragments from genomes is valuable for both basic and applied research, such as the construction of synthetic genomes, and the expression of biosynthetic gene clusters (BGCs) for natural product discovery. Here, we repo
Externí odkaz:
https://doaj.org/article/163145bcd8fc4ece8de09a8f9c10aeb9
Publikováno v:
Heliyon, Vol 10, Iss 6, Pp e26967- (2024)
DNA synthesis and assembly primarily revolve around the innovation and refinement of tools that facilitate the creation of specific genes and the manipulation of entire genomes. This multifaceted process encompasses two fundamental steps: the synthes
Externí odkaz:
https://doaj.org/article/84f80eb7855544ee9eaf2a23111788be
Autor:
Sarah Wiechers, Lara Marie Kösters, Dietmar Quandt, Thomas Borsch, Susann Wicke, Kai Frank Müller
Publikováno v:
Methods in Ecology and Evolution, Vol 14, Iss 3, Pp 799-805 (2023)
Abstract DNA barcoding aids in rapidly identifying specimens in various contexts by comparing short stretches of DNA to a reference database. Barcoding initiatives generate large reference databases of carefully curated high‐quality sequences to ma
Externí odkaz:
https://doaj.org/article/0aae0be4d273481e99ea5cf6dcfc5653
Publikováno v:
Journal of Low Power Electronics and Applications, Vol 14, Iss 1, p 9 (2024)
In this work, we present an efficient Processing in MRAM-Accelerated De Bruijn Graph-based DNA Assembly platform, named PANDA, based on an optimized and hardware-friendly genome assembly algorithm. PANDA is able to assemble large-scale DNA sequence d
Externí odkaz:
https://doaj.org/article/ed2d82633fb641e7addfdeb2ef98229e
Publikováno v:
Journal of Fungi, Vol 10, Iss 1, p 34 (2024)
For more than a thousand years, Aspergillus oryzae has been used in traditional culinary industries, including for food fermentation, brewing, and flavoring. In recent years, A. oryzae has been extensively used in deciphering the pathways of natural
Externí odkaz:
https://doaj.org/article/86a3ddeb3a3149cc92e5c8377438f852
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