Single Methylation Sensitive Restriction Endonuclease-Based Cascade Exponential Amplification Assay for Visual Detection of DNA Methylation at Single-Molecule Level.

Autor: Pataer P; Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China., Zhang P; Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China., Li Z; Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China.

Publikováno v: Analytical chemistry [Anal Chem] 2024 Aug 13; Vol. 96 (32), pp. 13335-13343. Date of Electronic Publication: 2024 Aug 02.

High-throughput direct screening of restriction endonuclease using a microfluidic fluorescence-activated drop sorter based on the SOS response in Escherichia coli .

Autor: Zhang Y; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. yizhe_zhang@post.harvard.edu., Agresti JJ; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. yizhe_zhang@post.harvard.edu., Zheng Y; New England BioLabs, Inc., Ipswich, MA 01938, USA., Weitz DA; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. yizhe_zhang@post.harvard.edu.; Department of Physics, Harvard University, Cambridge, MA 02138, USA.

Publikováno v: The Analyst [Analyst] 2024 Jun 24; Vol. 149 (13), pp. 3575-3584. Date of Electronic Publication: 2024 Jun 24.

Golden EGG, a simplified Golden Gate cloning system to assemble multiple fragments.

Autor: Biró, János Barnabás^1,2 (AUTHOR), Kecskés, Kristóf^1,3 (AUTHOR), Szegletes, Zita¹ (AUTHOR), Güngör, Berivan^1,2 (AUTHOR), Wang, Ting^1,4 (AUTHOR), Kaló, Péter^1,2 (AUTHOR), Kereszt, Attila¹ (AUTHOR) kereszt.attila@brc.hu

Publikováno v: Scientific Reports. 10/25/2024, Vol. 14 Issue 1, p1-12. 12p.

IMPRESS: Improved methylation profiling using restriction enzymes and smMIP sequencing, combined with a new biomarker panel, creating a multi-cancer detection assay.

Autor: Vandenhoeck J; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium., Neefs I; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium., Vanpoucke T; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium., Ibrahim J; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium., Suls A; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium., Peeters D; Department of Pathology, Antwerp University Hospital and University of Antwerp, Edegem, Belgium., Schepers A; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium., Hoischen A; Department of Human Genetics and Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre, Nijmegen, the Netherlands.; Department of Internal Medicine and Radboud Centre for Infectious Diseases (RCI), Radboud University Medical Centre, Nijmegen, the Netherlands., Fransen E; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium., Peeters M; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium., Van Camp G; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium., Op de Beeck K; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium. ken.opdebeeck@uantwerpen.be.; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium. ken.opdebeeck@uantwerpen.be.

Publikováno v: British journal of cancer [Br J Cancer] 2024 Oct; Vol. 131 (7), pp. 1224-1236. Date of Electronic Publication: 2024 Aug 24.

Dependence of post-segregational killing mediated by Type II restriction-modification systems on the lifetime of restriction endonuclease effective activity.

Autor: Kozlova S; Skolkovo Institute of Science and Technology, Center for Molecular and Cellular Biology, Moscow, Russia., Morozova N; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia., Ispolatov Y; Physics Department, University of Santiago of Chile, Center for Interdisciplinary Research in Astrophysics and Space Science, Santiago, Chile., Severinov K; Waksman Institute for Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, New Jersey, USA.; Institute of Gene Biology, Moscow, Russia.

Publikováno v: MBio [mBio] 2024 Aug 14; Vol. 15 (8), pp. e0140824. Date of Electronic Publication: 2024 Jul 09.