Zobrazeno 1 - 10
of 81
pro vyhledávání: '"D.A. Veal"'
Publikováno v:
Journal of Applied Microbiology
Aims: Fluorescence in situ hybridization (FISH) has been proposed for species-specific detection, and viability determination of Cryptosporidium parvum oocysts. FISH-based viability determination depends on rRNA decay after loss of viability. We exam
Publikováno v:
Journal of Applied Microbiology. 94:936-945
Aims:Pseudomonas spp. are considered the most important milk spoilage organisms. Here we describe development of a fluorescence in situ hybridization (FISH) probe specific for detection and enumeration of Pseudomonas spp. in milk. Methods and Results
Publikováno v:
Journal of Immunological Methods. 243:191-210
Large numbers of microbiological samples are analysed annually using traditional culture-based techniques. These techniques take hours to days to yield a result, are tedious and are not suitable for non-culturable microorganisms. Further, culture-bas
Autor:
Peter Karuso, D.A. Veal, A. D. Davison, J J French, Nanda Altavilla, Amanda Nouwens, Michael R. Gillings, Daniel Jardine
Publikováno v:
Scopus-Elsevier
The biphenyl degradation pathway of Sphingomonas paucimobilis BPSI-3 was investigated using a degradation-deficient mutant generated by 1-methyl-3-nitro-1-nitrosoguanidine (NTG) mutagenesis. The mutant, designated AN2, was confirmed as originating fr
Publikováno v:
Water Research. 33:1611-1617
Routine detection of Cryptosporidium oocysts and Giardia cysts depend on immunofluorescence assays (IFA) employing fluorescently labeled monoclonal antibodies. Commercially available mAbs used for the detection of Cryptosporidium oocysts are of the I
Publikováno v:
Journal of Applied Microbiology. 84:627-632
Grab samples of drinking water collected from reservoirs and from creeks flowing over pristine land, farmland or land having mixed use were analysed for their physicochemical and microbiological characteristics. A significant difference between sites
Publikováno v:
Biological Procedures
Biological Procedures Online, Vol 1, Iss 1, Pp 40-47 (1998)
Scopus-Elsevier
Biological Procedures Online, Vol 1, Iss 1, Pp 40-47 (1998)
Scopus-Elsevier
Amplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types (1). DNA is also suitabl
Publikováno v:
Letters in Applied Microbiology. 25:316-320
A simple method is described for the evaluation and quality control of Cryptosporidium-specific antibodies used in monitoring environmental water samples. Purified oocysts were fluorescently labelled with a test antibody at the appropriate concentrat
Publikováno v:
Cytometry. 29:147-154
Fluorescent labelling methods for detecting microorganisms in water have limited sensitivity partly due to the natural autofluorescence from environmental particles. The aim of this study was to examine the autofluorescence of water samples to determ
Publikováno v:
Scopus-Elsevier
A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (