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Development of Conventional and Real-Time Nucleic Acid Sequence-Based Amplification Assays for Detection of Chlamydophila pneumoniae in Respiratory Specimens

Autor: Margaretha Ieven, D. Ursi, T. Beck, Herman Goossens, Katherine Loens, M. Overdijk, Peter Sillekens

Publikováno v: Journal of clinical microbiology

Isothermal nucleic acid sequence-based amplification (NASBA) was applied to the detection of Chlamydophila pneumoniae 16S rRNA by using the NucliSens basic kit (bioMérieux, Boxtel, The Netherlands). The assay was originally developed as a conventio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30c3869e5b22cd48998ddfbf2fa0355b
https://doi.org/10.1128/jcm.44.4.1241-1244.2006

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Two Quality Control Exercises Involving Nucleic Acid Amplification Methods for Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and Carried Out 2 Years Apart (in 2002 and 2004)

Autor: T. Beck, S. R. Pattyn, Margareta Ieven, Katherine Loens, D. Ursi, H. Goossens

Publikováno v: Journal of Clinical Microbiology. 44:899-908

The quality performance of laboratories for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae by two quality control (QC) exercises with a 2-year interval was investigated. For the 2002 QC exercise, specimens were spiked with M. pne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::591dff060043c8ca41e6f94dbf69d939
https://doi.org/10.1128/jcm.44.3.899-908.2006

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Molecular Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections

Autor: Margareta Ieven, Herman Goossens, D. Ursi, Katherine Loens

Publikováno v: Journal of clinical microbiology

Mycoplasma pneumoniae is responsible for 10 to 20% of the cases of community-acquired pneumonia and has been associated with acute exacerbations of asthma ([22][1]). M. pneumoniae is also implicated in mild acute respiratory infections, such as sore

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::921bad3903f72421deee2b9489d4b768
https://doi.org/10.1128/jcm.41.11.4915-4923.2003

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Importance of Methodology in Determination of Chlamydia pneumoniae Seropositivity in Healthy Subjects and in Patients with Coronary Atherosclerosis

Autor: C.J. Vrints, Margareta Ieven, L. Van Renterghem, D. Ursi, Vicky Y. Hoymans, Johan Bosmans, Rudolf P. Mak, Floris L. Wuyts

Publikováno v: Journal of clinical microbiology

Enzyme immunoassays (EIAs) for the detection of Chlamydia pneumoniae antibodies were compared to the microimmunofluorescence (MIF) test, the reference method. Furthermore, we assessed the hypothesis that a possible relationship between Chlamydia pneu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8eb710cbd4753e941171d5d3e3e68bb6
https://doi.org/10.1128/jcm.41.9.4049-4053.2003

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Improved Detection of Rhinoviruses by Nucleic Acid Sequence-Based Amplification after Nucleotide Sequence Determination of the 5′ Noncoding Regions of Additional Rhinovirus Strains

Autor: D. Ursi, Herman Goossens, P. Sillekens, Katherine Loens, P. Oudshoorn, C. de Laat, Margareta Ieven

Publikováno v: Journal of clinical microbiology

The isothermal nucleic acid sequence-based amplification (NASBA) system was applied for the detection of rhinoviruses using primers targeted at the 5′ noncoding region (5′ NCR) of the viral genome. The nucleotide sequence of the 5′ NCRs of 34 r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1bba4877f5ea24001498250d3f25782
https://doi.org/10.1128/jcm.41.5.1971-1976.2003

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SYNTHESIS AND BIOLOGICAL EVALUATION OF SMALL MOLECULES DERIVATIVES OF THE NATURAL HISTONE DEACETYLASE INHIBITOR FR235222

Autor: Randino, Rosario, Cini, Elena, Anna Maria, D�Ursi, Petricci, Elena, Rodriquez, Manuela

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1820::759e9bbd6c4b66d33db7a3b6d0a54733
http://hdl.handle.net/11365/999252

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Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs

Autor: Herman Goossens, D. Ursi, Margareta Ieven, Katherine Loens

Publikováno v: Journal of microbiological methods

Nucleic acids were extracted from 215 throat swabs from patients with community-acquired pneumonia by the manual Boom extraction, the NucliSens miniMAG and the Qiagen DNA blood kit and amplified respectively by in-house real-time NASBA, NucliSens Eas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::325cdf4f80ab92109c82984cc4e773eb
https://doi.org/10.1016/j.mimet.2007.11.008

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Construction of an internal control for the detection ofChlamydia pneumoniaeby PCR

Autor: Herman Goossens, Margareta Ieven, D. Ursi, H. P. Van Bever

Publikováno v: Molecular and cellular probes

For the detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in respiratory samples, an internal control was constructed to monitor the efficiency of amplification in each reaction. The internal control was designed in a way that the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90c79a29b96cb3e6fa82a4c7309d5093
https://doi.org/10.1006/mcpr.1998.0180

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