Zobrazeno 1 - 10
of 86
pro vyhledávání: '"D. Tripier"'
Publikováno v:
Analytical Biochemistry. 210:366-373
Evaluation of the yield of expression of exogeneous protein in transformed Escherichia coli cells by means of one-dimensional SDS-PAGE often leads to overestimation and miscalculation. For example, it is possible that proteins of similar size comigra
Publikováno v:
FEBS Letters. 292:205-209
Isolated interphase lamin C, obtained from Ehrlich ascites tumor cells, was digested by Lys-C endoproteinase, the resulting peptides separated by reversed-phase HPLC and subjected to microsequencing in order to identify phosphorylation sites in inter
Autor:
Harald Stein, Detlef Schuppan, Hermann Herbst, Michael Hummel, M. Just, D Tripier, Horst Dürkop
Publikováno v:
Journal of Biological Chemistry. 266:17326-17332
We characterized cDNA clones specific for the extracellular matrix glycoprotein undulin. Two sets of cDNA clones were isolated from a human placental lambda gt11 expression library and from a rhabdomyosarcoma cell line encoding two partially identica
Publikováno v:
Analytica Chimica Acta. 249:271-278
In evaluating the downstream processing of genetic engineered human insulin there has been no plausible correlation between the possible yield (determined via sodium dodecyl sulphate polyacrylamide gel electrophoresis) and the actual yield, i.e., the
Autor:
E. Klaus, P. Habermann, Brigitte Hušák, V. Malerczyk, P. Crause, H. Grötsch, M. Hropot, G. Apidopoulos, D. Tripier
Publikováno v:
Hämostaseologie. 11:129-131
ZusammenfassungDie Pharmakodynamik und Pharmakokinetik von rekombinantem DNA-Hirudin HBW 023 wurde an Rhesus-Affen in Dosen von 0,1; 0,5; 1,0 und 10,0 mg/kg nach einmaliger intravenöser oder subkutaner Applikation untersucht. HBW 023 zeigte eine dos
Publikováno v:
Connective tissue research. 28(4)
Extracts of cartilage have been reported to inhibit many serine proteinases and metalloenzymes. Such inhibition may be important in protecting cartilage against degradation by chondrocytic proteinases such as collagenase, stromelysin and by leukocyti
Publikováno v:
FEBS letters. 292(1-2)
Isolated interphase lamin C, obtained from Ehrlich ascites tumor cells, was digested by Lys-C endoproteinase, the resulting peptides separated by reversed-phase HPLC and subjected to microsequencing in order to identify phosphorylation sites in inter
Publikováno v:
Biochimica et biophysica acta. 1076(1)
Purified pea chloroplast NADP-malate dehydrogenase (S)-malate: NADP+ oxidoreductase, EC 1.1.1.82) was digested with trypsin and the resulting peptides were separated by HPLC and sequenced. Together with the information from earlier work (Fickenscher,
Publikováno v:
Molecular microbiology. 4(5)
Summary The fumarate reductase operon of Wolinella succinogenes is made up of three structural genes (frd-CAB). The frdC gene was located next to the promoter region and identified as the cytochrome b structural gene encoding 256 amino acid residues.
Publikováno v:
Applied and environmental microbiology. 56(1)
An aminotransferase capable of transaminating 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid to L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG), was purified to apparent h