Structural and biochemical characterization of the relaxosome auxiliary proteins encoded on the Bacillus subtilis plasmid pLS20

Autor: Isidro Crespo, Nerea Bernardo, Anna Cuppari, Barbara M. Calisto, Jorge Val-Calvo, Andrés Miguel-Arribas, Wilfried J.J. Meijer, Xavi Carpena, Fernando Gil-Ortiz, Marc Malfois, D. Roeland Boer

Publikováno v: Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 757-765 (2022)

Bacterial conjugation is an important route for horizontal gene transfer. The initial step in this process involves a macromolecular protein-DNA complex called the relaxosome, which in plasmids consists of the origin of transfer (oriT) and several pr

Externí odkaz: https://doaj.org/article/f9e7930838b14d2e9a0dfb7766df5721

Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20

Autor: Wilfried J. J. Meijer, D. Roeland Boer, Saúl Ares, Carlos Alfonso, Fernando Rojo, Juan R. Luque-Ortega, Ling Juan Wu

Publikováno v: Frontiers in Molecular Biosciences, Vol 8 (2021)

Bacterial conjugation is the main horizontal gene transfer route responsible for the spread of antibiotic resistance, virulence and toxin genes. During conjugation, DNA is transferred from a donor to a recipient cell via a sophisticated channel conne

Externí odkaz: https://doaj.org/article/4f174db42c914c8583975c4f1954bfba

Structural basis of direct and inverted DNA sequence repeat recognition by helix–turn–helix transcription factors

Autor: Raul Fernandez-Lopez, Raul Ruiz, Irene del Campo, Lorena Gonzalez-Montes, D Roeland Boer, Fernando de la Cruz, Gabriel Moncalian

Publikováno v: Nucleic Acids Research, 2022, Vol. 50, No. 20

Some transcription factors bind DNA motifs containing direct or inverted sequence repeats. Preference for each of these DNA topologies is dictated by structural constraints. Most prokaryotic regulators form symmetric oligomers, which require operator

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2745812f6fbf9204e0b8ae9025edbd41
https://doi.org/10.1093/nar/gkac1024

Discovery of a new family of relaxases in Firmicutes bacteria.

Autor: Gayetri Ramachandran, Andrés Miguel-Arribas, David Abia, Praveen K Singh, Isidro Crespo, César Gago-Córdoba, Jian An Hao, Juan Roman Luque-Ortega, Carlos Alfonso, Ling J Wu, D Roeland Boer, Wilfried J J Meijer

Publikováno v: PLoS Genetics, Vol 13, Iss 2, p e1006586 (2017)

Antibiotic resistance is a serious global problem. Antibiotic resistance genes (ARG), which are widespread in environmental bacteria, can be transferred to pathogenic bacteria via horizontal gene transfer (HGT). Gut microbiomes are especially apt for

Externí odkaz: https://doaj.org/article/0bb180600a96476abef56dfac9445a0c

Architecture of DNA elements mediating ARF transcription factor binding and auxin-responsive gene expression in Arabidopsis

Autor: Simon Lindhoud, Isidro Crespo, Elmar van der Wijk, Dolf Weijers, Victor G. Levitsky, Mattia Fontana, Victoria V. Mironova, Yana Sizentsova, D. Roeland Boer, Keita Tanaka, Johannes Hohlbein, Alejandra Freire-Rios

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 39
Proceedings of the National Academy of Sciences of the United States of America, 117(39), 24557-24566
Proceedings of the National Academy of Sciences of the United States of America

Significance The plant hormone auxin controls many aspects of growth and development. It does so by changing the activity of AUXIN RESPONSE FACTOR (ARF) proteins that recognize specific DNA elements in plant genes and switch gene expression on or off

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6098cb5549086acc8a1f221d6bd78d3
https://doi.org/10.1073/pnas.2009554117