Zobrazeno 1 - 10
of 33
pro vyhledávání: '"D. R. Muhandiram"'
Publikováno v:
Journal of Biomolecular NMR. 27:175-179
A triple resonance NMR experiment is presented for the simultaneous recording of HNCA and HNCO data sets on 15N, natural abundance 13C samples. The experiment exploits the fact that transfers of magnetization from 15N to 13CO and from 15N to 13Cα (a
Autor:
R.E.D McClung, D. R. Muhandiram, G. H. M. Aarts, D. M. Heinekey, William A. G. Graham, Gong-Yu Kiel, J. G. A. Reuvers, Josef Takats, I. M. Saez
Publikováno v:
Organometallics. 21:2687-2704
Multisite magnetization transfer (MMT) studies of metal migration around the C7H7 rings in (η3-C7H7)Re(CO)4 (1), (η3-C7H7)Os(CO)3SnPh3 (2), (η3-C7H7)Re(CO)3PMe3 (3), (η5-C7H7)Re(CO)3 (4), (η5-C7H7)Fe(CO)2SnPh3 (5), (η5-C7H7)Os(CO)2SnPh3 (6), an
Publikováno v:
Journal of the American Chemical Society. 124:6439-6448
New pulse sequences are presented for the measurement of the relaxation of deuterium double quantum, quadrupolar order, and transverse antiphase magnetization in (13)CH(2)D methyl groups of (15)N-, (13)C-labeled, fractionally deuterated proteins. Tog
Publikováno v:
Journal of the American Chemical Society. 122:7059-7071
Experiments for measuring chemical shift anisotropy (CSA) cross-correlated spin relaxation between pairs of carbonyl spins in 15 N, 13 C-labeled proteins based on differences in relaxation rates of double- and zero-quantum carbonyl coherences are pre
Autor:
Daniela Rotin, Voula Kanelis, Julie D. Forman-Kay, Logan W. Donaldson, Lewis E. Kay, D. R. Muhandiram
Publikováno v:
Journal of Biomolecular NMR. 16:253-259
Many protein-protein interactions involve amino acid sequences containing proline-rich motifs and even polyproline stretches. The lack of amide protons in such regions complicates assignment, since 1HN-based triple-resonance assignment strategies can
Publikováno v:
Journal of Molecular Biology. 294:967-979
The ribonucleoprotein (RNP) domain is one of the most common eukaryotic protein domains, and is found in many proteins involved in recognition of a wide variety of RNAs. Two structures of RNA complexes of human U1A protein have revealed important asp
Autor:
D. R. Muhandiram, Mitsuhiko Ikura, Tetsuro Kokubo, Rieko Ishima, Yoshihiro Nakatani, Lewis E. Kay, Kit I. Tong, Dingjiang Liu, Stefan Bagby
Publikováno v:
Cell. 94:573-583
General transcription factor TFIID consists of TATA box–binding protein (TBP) and TBP-associated factors (TAF II s), which together play a central role in both positive and negative regulation of transcription. The N-terminal region of the 230 kDa
Publikováno v:
Journal of Biomolecular NMR. 11:307-318
Deuterium decoupled, triple resonance NMR spectroscopy was used to analyze complexes of 2H, 15N, 13C labelled intact and (des2-7) trp repressor (delta 2-7 trpR) from E. coli bound in tandem to an idealized 22 basepair trp operator DNA fragment and th
Autor:
Philip E. Johnson, Lewis E. Kay, D. R. Muhandiram, Lawrence P. McIntosh, Ouwen Zhang, Daiwen Yang
Publikováno v:
Journal of Biomolecular NMR. 10:283-288
15 N, 13 C, 2 H-labeled proteins; 2 H spin relaxation; 15 N, NH correlations Summary A triple-resonance pulse scheme is described which records 15 N, NH correlations of residues that immediately follow a methyl-containing amino acid. The experiment m
Publikováno v:
Journal of Biomolecular NMR. 9:409-422
Pulse sequences are presented for the measurement of3JC′Cγ and3JNCγ scalar couplings for allCγ containing residues in15N,13C uniformly labeled proteins. The methodsdescribed are based on quantitative J correlation spectroscopy pioneered byBax an