Výsledky vyhledávání - "D. M. Pérez-Filgueira"

Protection of Mice against Challenge with Foot and Mouth Disease Virus (FMDV) by Immunization with Foliar Extracts from Plants Infected with Recombinant Tobacco Mosaic Virus Expressing the FMDV Structural Protein VP1

Autor: Thomas Jack Morris, A.M. Sadir, M.V. Borca, Consuelo Carrillo, D. M. Pérez Filgueira, N. Robertson, Andrés Wigdorovitz

Publikováno v: Virology. 264(1):85-91

A tobacco mosaic virus (TMV)-based vector has been used to express in plants the complete open reading frame coding for VP1, the major immunogenic protein of foot and mouth disease virus (FMDV). In vitro RNA transcripts were inoculated into Nicotiana

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa19b370b54af232adb444baa7c3b59b

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DIVA diagnostic of Aujeszky's disease using an insect-derived virus glycoprotein E

Autor: D. M. Pérez-Filgueira, Maria del Carmen Nuñez, I. Sánchez-Ramos, J.M. Escribano, Silvia Gómez-Sebastián, E. Gomez-Casado, E. Tabarés

Publikováno v: Repositorio de Resultados de Investigación del INIA
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
INIA: Repositorio de Resultados de Investigación del INIA

Commercial vaccines against Aujeszky's disease are mainly formulated using deleted versions of attenuated or inactivated Pseudorabies virus (PRV) particles lacking of the structural glycoprotein E (gE). Complementary diagnostic assays used to differe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30526c1567e02d9afe96968d87c3c7f9
http://hdl.handle.net/20.500.12792/1817

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Optimization and Validation of Recombinant Serological Tests for African Swine Fever Diagnosis Based on Detection of the p30 Protein Produced in Trichoplusia ni Larvae

Autor: D. M. Pérez-Filgueira, P. Resino-Talaván, Fernando González-Camacho, J.M. Escribano, Carmina Gallardo, Esther Blanco, Covadonga Alonso, E. Gómez-Casado

We describe the validation of an enzyme-linked immunosorbent assay (ELISA) and confirmatory immunoblotting assays based on a recombinant p30 protein (p30r) produced in insect larvae using a baculovirus vector. Such validation included the following (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::563e3e863cbe88d25240760243b3fbff
https://europepmc.org/articles/PMC1594705/

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Preserved antigenicity of HIV-1 p24 produced and purified in high yields from plants inoculated with a tobacco mosaic virus (TMV)-derived vector

Autor: Thomas Jack Morris, Charles E. Wood, Manuel V. Borca, Brad P. Brayfield, Saul Phiri, D. M. Pérez-Filgueira

Publikováno v: Repositorio de Resultados de Investigación del INIA
INIA: Repositorio de Resultados de Investigación del INIA
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA

Production of structural proteins from foot-and-mouth disease virus (FMDV) and bovine herpes virus (BHV-1) in Nicotiana benthamiana through the use of a tobacco mosaic virus-based vector (TMV-30B) has been reported previously. The development of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9602e2d80b489cdfcd4e63c3f83e5a93
http://hdl.handle.net/20.500.12792/3448

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Passive protection to bovine rotavirus (BRV) infection induced by a BRV VP8* produced in plants using a TMV-based vector

Autor: Karina Trono, Marina Valeria Mozgovoj, A. Wigdorovitz, F. Fernandez, Consuelo Carrillo, M.J. Dus Santos, T. J. Morris, Manuel V. Borca, Viviana Parreño, D. M. Pérez Filgueira, Lorne A. Babiuk

We have previously reported on the use of a tobacco mosaic virus (TMV) vector TMV-30B to express foreign viral antigens for use as experimental immunogens. Here we describe the development of an improved TMV-30B vector that adds a sequence of 7 histi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7238bc295c565fff4af20da479c93372
http://hdl.handle.net/20.500.12792/3394

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Bovine herpes virus gD protein produced in plants using a recombinant tobacco mosaic virus (TMV) vector possesses authentic antigenicity

Autor: M. P. Del Medico Zajac, A.M. Sadir, M. Puntel, Patricia Ines Zamorano, Sonia Alejandra Romera, M. G. Dominguez, Oscar Taboga, Thomas Jack Morris, D. M. Pérez Filgueira, Manuel V. Borca

A tobacco mosaic virus (TMV)-based vector was utilized for expression of a cytosolic form of the bovine herpesvirus type 1 (BHV-1) protein glycoprotein D (gDc). Nicotiana benthamiana plants were harvested 7 days after inoculation with RNA transcripts

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbc2dd4ae907c816195b31d24fc64785
http://hdl.handle.net/20.500.12792/3395

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