Zobrazeno 1 - 10
of 64
pro vyhledávání: '"D. L. Garner"'
Publikováno v:
Small Ruminant Research. 55:239-243
A major obstacle in successful cooled semen storage is the reactive oxygen species generated in the media by the sperm cells. Recently, many reports document the antioxidant, anti-carcinogenic and anti-mutagenic properties of flavonoids when used in
Publikováno v:
Theriogenology. 56:31-40
Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, the
Publikováno v:
Reproductive Toxicology. 15:5-10
Fluorescent assessment of cellular integrity and mitochondrial function by flow cytometry can provide a rapid and precise means of determining the functional status of large numbers of spermatozoa. In the present study, rat sperm viability was assess
Publikováno v:
Reproduction in Domestic Animals. 34:399-404
Contents Eight bull ejaculates were split to evaluate the effects of glycerol on sperm organelle function. Although glycerol protects sperm membranes during cryopreservation, preliminary data suggested that glycerol was detrimental to sperm organelle
Autor:
D. L. Garner, C.A. Thomas
Publikováno v:
Molecular Reproduction and Development. 53:222-229
The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differ
Publikováno v:
Biology of Reproduction. 58:786-793
Flow cytometry was used to compare the functional status of fluorescently stained sperm organelles from 12 Holstein bulls after storage for 24 h at 5 degrees C and after cryopreservation. The organelle-specific stains, SYBR-14 and LysoTracker Green D
Publikováno v:
Reproduction in Domestic Animals. 32:279-283
Publikováno v:
Biology of Reproduction. 57:1401-1406
Mitochondrial function and sperm viability were quantified in samples of cryopreserved bovine spermatozoa from 12 bulls using fluorometric techniques. The active mitochondria of the spermatozoa were fluorescently stained using three different fluorop
Publikováno v:
Biology of Reproduction. 56:991-998
The combination of specific fluorometric staining and flow cytometry provides a rapid and precise means of assessing the functional status of cells. We sought to utilize this approach to quantify two important seminal characteristics, acrosomal integ
Publikováno v:
Theriogenology. 47:521-530
The accuracy and repeatability of freezing rates and effects of evaporation were examined using a new cryomicroscope system to establish its usefulness in assessing the development of cryopreservation protocols for bovine semen. Post-thaw sperm plasm