Zobrazeno 1 - 10
of 66
pro vyhledávání: '"D. J. Bolt"'
Autor:
D. J. Bolt, Richard D. Palmiter, Vernon G. Pursel, Carl A. Pinkert, K. F. Miller, Robert E Hammer, Ralph L. Brinster
Publikováno v:
Bioscientifica Proceedings.
Recent research clearly shows that fusion genes can be microinjected into a pronucleus of an ovum and integrate into the pig genome. Animals with such fusion genes are called 'transgenic'. The percentage of injected ova that developed into transgenic
Publikováno v:
Journal of Animal Science. 72:1242-1246
Fatty acid profiles and cholesterol content of whole-carcass ground tissue were compared from 26 transgenic (T) pigs expressing a bovine growth hormone gene (bGH) to 26 sibling control (C) pigs. All pigs were fed a common diet and were slaughtered at
Publikováno v:
Journal of Animal Science. 71:3409-3413
Prepuberal gilts were injected with PMSG to determine whether expression of a bovine growth hormone (bGH) transgene inhibited preovula- tory maturation of ovarian follicles. Seven transgenic (TG) gilts of line 3706, which expresses a mouse metallothi
Autor:
Ted H. Elsasser, Kelly E. Mayo, Ralph L. Brinster, D. J. Bolt, K. F. Miller, Richard D. Palmiter, Richard R. Behringer, Caird E. Rexroad
Publikováno v:
Journal of Animal Science. 69:2995-3004
Chimeric genes containing either the mouse transferrin (Trf) enhancer/promoter fused to the structural sequences encoding bovine growth hormone (GH) or the mouse albumin (Alb) enhancer/promoter fused to the gene for human growth hormone-releasing fac
Autor:
D. J. Bolt, H.D. Guthrie
Publikováno v:
Domestic Animal Endocrinology. 7:83-91
This experiment was conducted to determine the changes in secretion of LH, FSH, estrogen and progesterone during follicle maturation. Ovaries were recovered from 11 non-treated (control) gilts, three on day 13, four on day 16, and four on day 19 of t
Publikováno v:
Archives of andrology. 29(1)
Bovine sperm plasma membranes were extracted with deoxycholate and subjected to two-dimensional gel electrophoresis. Proteins were visualized either by silver staining or autoradiography using 125I. When 1.5-2.0 micrograms of protein extract was appl
Autor:
D.W. Caldwell, D. J. Bolt
Publikováno v:
Domestic animal endocrinology. 9(1)
Progress to understand mechanisms that regulate inhibin secretion and action in farm animals has been handicapped by the shortage of simple, accurate assay methods to quantify inhibin in circulation. RIA would seem to provide the needed quantitative
Autor:
Richard D. Palmiter, K. F. Miller, Vernon G. Pursel, H.D. Guthrie, Ralph L. Brinster, D. J. Bolt
Publikováno v:
Domestic animal endocrinology. 8(3)
Chronic supraphysiological blood levels of growth hormone (GH) may retard sexual maturation in swine. Pigs used in this study included four founder transgenic pigs (two gilts and two boars) expressing a mouse transferrin (TF) promoter fused to a bovi
Publikováno v:
Journal of animal science. 68(11)
This experiment was conducted to compare the ability of USDA porcine FSH-B-1 (pFSH), USDA porcine LH-B-1 (pLH), and pregnant mare's serum gonadotropin (PMSG) to grow large follicles and induce granulosal cell aromatase activity in prepuberal gilts. T
Publikováno v:
Journal of reproduction and fertility. Supplement. 40
Recent research clearly shows that fusion genes can be microinjected into a pronucleus of an ovum and integrate into the pig genome. Animals with such fusion genes are called 'transgenic'. The percentage of injected ova that developed into transgenic