Zobrazeno 1 - 10
of 67
pro vyhledávání: '"D. G. Whittingham"'
Autor:
S. J. Fuller, D. G. Whittingham
Publikováno v:
Reproduction. 108:139-145
Attempts to freeze mouse spermatozoa in liquid nitrogen (-196 degrees C) have met with limited success. In an attempt to identify the factor(s) that damage mouse spermatozoa during cryopreservation, the effect of slow cooling to 4 degrees C was exami
Publikováno v:
Reproduction. 93:71-79
Collagen gels containing isolated primary follicles devoid of other ovarian tissue were transferred beneath the kidney capsule of 3 types of female recipients: cycling, ovariectomized and hypogonadal, known to have different circulating concentration
Publikováno v:
Reproduction. 90:321-327
Isolated primary mouse follicles can be frozen successfully and thawed in the presence of 1.5 m-DMSO. Similar proportions of freshly collected and frozen-thawed primary follicles undergo folliculogenesis in the absence of other ovarian tissue. Some o
Publikováno v:
Human Reproduction. 5:217-220
The culture of human preimplantation embryos from the 1-cell to the morula/blastocyst stage of development is not satisfactory at present. The success of various IVF laboratories ranges from 18 to 23%, therefore there is a requirement for improvement
Autor:
S J, Fuller, D G, Whittingham
Publikováno v:
Molecular reproduction and development. 46(3)
Previously we showed that70% of mouse spermatozoa cooled slowly from 37 degrees C to 4 degrees C and warmed have undergone capacitation-like changes as examined by a chlortetracycline staining assay. These membrane changes are reflected in the abilit
Autor:
L, Scott, D G, Whittingham
Publikováno v:
Molecular reproduction and development. 43(3)
One-cell embryos from some inbred and random-bred mice, but not those derived from certain F1 hybrids, suffer from a block during in vitro development known as the two-cell block. This two-cell block can be overcome by removing glucose or inorganic p
Publikováno v:
Reproduction, fertility, and development. 8(6)
The cell cycle characteristics of mouse cumulus granulosa cells were determined before, during and following their expansion and mucification in vivo and in vitro. Cumulus-oocyte complexes (COC) were recovered from ovarian follicles or oviducts of pr
Publikováno v:
Human reproduction (Oxford, England). 10(9)
Pieces of marmoset ovary were frozen by slow cooling in 1.5 M dimethylsulphoxide. The follicles in fresh and frozen tissue were counted and examined for morphological appearance in stained serial sections. The proportion of normal follicles was simil
Publikováno v:
Human reproduction (Oxford, England). 9(9)
Mouse oocytes enclosed in cumulus cells were isolated from antral follicles at the germinal vesicle (GV) stage. They were stored in straws at -196 degrees C by a conventional mouse embryo freezing method using dimethylsulphoxide (1.5 M) as the cryopr