Zobrazeno 1 - 10 of 43 pro vyhledávání: '"D. G. Kozlov"'
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Preparation and Properties of the Recombinant Tenebrio molitor SerPH122—Proteolytically Active Homolog of Serine Peptidase
Autor: P. I. Akentyev, I. Yu. Filippova, Nikita I. Zhiganov, Valeriia F. Tereshchenkova, I. I. Gubaidullin, N. V. Belyaeva, D. G. Kozlov, Elena N. Elpidina
Publikováno v: Applied Biochemistry and Microbiology. 57:579-585
Pseudoenzymes are homologs of active enzymes that have amino acid substitutions in the active center and, therefore, usually do not possess enzymatic activity. In this work, a recombinant proprotein (proSerPH122) of the homolog of serine peptidases o
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::06e509a8066584f52b452e38fe0b571f
https://doi.org/10.1134/s0003683821050161
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4
Highly Active Modified Variants of Recombinant Phospholipase А2 from Streptomyces violaceoruber for Effective Expression in Yeasts
Autor: F. A. Klebanov, S. E. Cheperegin, E. P. Sannikova, A. V. Malysheva, D. G. Kozlov
Publikováno v: Applied Biochemistry and Microbiology. 56:770-778
The ability of yeast to produce highly active variants of the recombinant phospholipase PLA2 is confirmed. Highly active variants were based on an original enzyme from the Streptomyces violaceoruber А-2688 strain. Various approaches to reduce enzyme
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e83166acbea6e45502b5401ca312b32c
https://doi.org/10.1134/s0003683820070029
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5
Properties and Biotechnological Application of Mutant Derivatives of the Mini-Intein PRP8 from Penicillium chrysogenum with Improved Control of C-Terminal Processing
Autor: F. A. Klebanov, S. E. Cheperegin, D. G. Kozlov, E. P. Sannikova
Publikováno v: Applied Biochemistry and Microbiology. 56:861-869
Mutant variants of mini-intein PRP8 from Penicillium chrysogenum (Int4b) with improved control of C-terminal processing are characterized. The studied variants can serve as the basis for the self-removal of polypeptide tags that can carry an affine l
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::059ba4db36ec1e70eaca1e04675b0deb
https://doi.org/10.1134/s0003683820080098
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6
Expression of Highly Active Bacterial Phospholipase A2 in Yeast Using Intein-Mediated Delayed Protein Autoactivation
Autor: D. G. Kozlov, S. E. Cheperegin, A. V. Malysheva, B. D. Efremov, I. I. Gubaidullin, E. P. Sannikova
Publikováno v: Applied Biochemistry and Biotechnology. 193:1351-1364
Phospholipase A2 (PLA2) has found extensive use in industry. However, recombinant PLA2 production in different expression systems is a difficult task because of its toxicity to cell membranes. We report here the development of an effective method for
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::80abc0ff80c82c07eb93f011b74a7376
https://doi.org/10.1007/s12010-020-03333-7
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7
Intranasal Human Recombinant Modified Glucagon-Like Peptide-1: High Antihyperglycemic Activity and Duration of Action in Mice
Autor: N. V. Rykalina, N. V. Bulushova, D. G. Kozlov, E. V. Askerova
Publikováno v: Bulletin of Experimental Biology and Medicine. 169:53-56
The study compared effectiveness of intranasal administration of glypin (human recombinant modified glucagon-like peptide-1) and reference drug Victoza in BALB/c mice. The minimum effective dose of intranasal glypin was 0.5 mg/kg, and a 2-fold elevat
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db348bf07ce8603505346102454ea605
https://doi.org/10.1007/s10517-020-04822-9
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8
Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii
Autor: I. I. Gubaidullin, P. I. Akentyev, D. G. Kozlov, Elena N. Elpidina, Valeriia F. Tereshchenkova, Nikita I. Zhiganov
Publikováno v: Biotekhnologiya. 36:78-86
A strain of the Komagataella kurtzmanii yeast, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of the pro-enzyme secretion was 20-50 mg/L. It was shown that, during secretion in yeast
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::015b80f84effa210affd3593b8410d82
https://doi.org/10.21519/0234-2758-2020-36-6-78-86
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9
Ubiquitin-Specific E. coli Proteinase Does Not Require the Obligatory Presence of Dipeptide GlyGly at Processing Site
Autor: D. G. Kozlov, E. P. Sannikova, S. E. Cheperegin
Publikováno v: Applied Biochemistry and Microbiology. 55:846-849
It is shown that, unlike eukaryotic ubiquitin-specific proteinases (deubiquitinating enzymes, DUBs), the E. coli deubiquitinase ElaD can process sites containing not only GlyGly but also GlyAla with equal efficiency. At the same time, the bacterial e
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::36a37831320724d3afbf1f5c4d3bdc79
https://doi.org/10.1134/s0003683819090060
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10
Specific Activity of Recombinant Modified Human Glucagon-Like Peptide 1
Autor: T. S. Gracheva, F. A. Klebanov, E. P. Sannikova, S. V. Yarotskii, I. A. Zalunin, V. L. Yurin, S. E. Cheperegin, N. V. Rykalina, E. V. Askerova, N. V. Bobkova, D. G. Kozlov, N. V. Bulushova, O. G. Tatarnikova
Publikováno v: Applied Biochemistry and Microbiology. 55:722-732
An1 original drug, Glypin, has been developed for the treatment of type-II human diabetes mellitus. Its active pharmaceutical substance is a completely biosynthetic, recombinant, modified, human glucagon-like peptide (rmGlp-1) obtained via culturing
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8c3032de0b944216c46243755eaba2f8
https://doi.org/10.1134/s0003683819070068
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