Výsledky vyhledávání

Antisense targeting of oxytocinergic hypothalamus neurons induces cytoplasmic triple helix-like immunoreactivity

Autor: G F, Jirikowski, J, Culman, J S, Lee, D, Celeda, K, Prüfer

Publikováno v: Cellular and molecular biology (Noisy-le-Grand, France).

Intracerebroventricular injections of oligonucleotide probes complementary to oxytocin mRNA are known to decrease systemic oxytocin levels. In this study we show that immunoreactive oxytocin in the magnocellular hypothalamic perikarya and in their ne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::081dffc2f121d4a6f54dd22f16513224
https://pubmed.ncbi.nlm.nih.gov/14995079

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TGF beta and bFGF synthesis and localization in Dupuytren's disease (nodular palmar fibromatosis) relative to cellular activity, myofibroblast phenotype and oncofetal variants of fibronectin

Autor: A, Berndt, H, Kosmehl, U, Mandel, U, Gabler, X, Luo, D, Celeda, L, Zardi, D, Katenkamp

Publikováno v: The Histochemical journal. 27(12)

Nodular palmar fibromatosis is a self-limited proliferation of fibro-/myofibroblasts associated with growth factor synthesis and abundant fibronectin extracellular matrix deposition. bFGF and TGF beta are potent modulators of fibro-/myofibroblast pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::22827b0aa7fd3fe2b1c51b4762d8016e
https://pubmed.ncbi.nlm.nih.gov/8789403

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Transcription specific differences visualized by fluorescence in situ hybridization pattern on interphase nuclei of different cell types

Autor: D, Celeda, P, Mühlig, A, Berndt, H, Kosmehl, G, Löber, K O, Greulich

Publikováno v: Cellular and molecular biology (Noisy-le-Grand, France). 41(8)

Application of a "formamide free" and thus "material preserving" in situ hybridization technique using the cDNA of the myf3 gene revealed the following results: Human rhabdomyosarcoma cells, characterized by a high expression of myf3 show intensive h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8ccdf3fe984cd5ce8831a86eabc2d61d
https://pubmed.ncbi.nlm.nih.gov/8747083

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Differential expression of fibronectin splice variants, oncofetal glycosylated fibronectin and laminin isoforms in nodular palmar fibromatosis

Autor: Detlef Katenkamp, Alexander Berndt, Hartwig Kosmehl, Ulla Mandel, R. Bohle, U. Gabler, D. Celeda

Publikováno v: University of Copenhagen

Summary The tissue formation process in nodular palmar fibromatosis (Morbus Dupuytren) was investigated by the demonstration of fibronectin splice variants (ED-A and ED-B fibronectin), de novo glycosylated fibronectin and laminin isoforms (A, M, B 1,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7434cc47c6daf4a2e3b9e5fa37812a2d
https://pubmed.ncbi.nlm.nih.gov/8822112

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Sense- and antisense-targeting of oxytocinergic systems in the rat hypothalamus

Autor: G F, Jirikowski, D, Celeda, M, Jantz, K, Prüfer, J S, Lee

Publikováno v: Advances in experimental medicine and biology. 395

3' phosphorothioate modified sense or antisense oligonucleotides to oxytocin transcripts were used for in vivo targeting of oxytocin (OT) neurons in the rat hypothalamus. Intracerebroventricular injections of antisense probe resulted in a loss of sys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0f79e5a2fe654c8433774ae894dcf6ff
https://pubmed.ncbi.nlm.nih.gov/8713952

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A rapid FISH technique for quantitative microscopy

Autor: F M, Haar, M, Durm, K, Aldinger, D, Celeda, M, Hausmann, H, Ludwig, C, Cremer

Publikováno v: BioTechniques. 17(2)

Results of quantitative microscopy for fluorescence in situ hybridization (FISH) signals with repetitive DNA probes (pUC 1.77 and D15Z1) are reported. A nonenzymatic hybridization technique was applied using fluorescein-12-dUTP labeled DNA probes and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4404153679b444d0490bb5761bc9ba3a
https://pubmed.ncbi.nlm.nih.gov/7980939

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PCR amplification and simultaneous digoxigenin incorporation of long DNA probes for fluorescence in situ hybridization

Autor: D, Celeda, U, Bettag, C, Cremer

Publikováno v: BioTechniques. 12(1)

Nonradioactive in situ hybridization has found widespread applications in cytogenetics. Basic requirements are DNA probes in sufficient amounts and of high specificity as well as a labeling protocol of good reproducibility. The PCR has been of fundam

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::be5ef80f5c08b3985f9a789bb6e050f6
https://pubmed.ncbi.nlm.nih.gov/1734931

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