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pro vyhledávání: '"D. Bruce Keech"'
Autor:
D. Bruce Keech
Publikováno v:
Pyruvate Carboxylase ISBN: 9781351076142
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::98f7a0d0781114c033e18c73da4e9c5c
https://doi.org/10.1201/9781351076142-1
https://doi.org/10.1201/9781351076142-1
Publikováno v:
Biochemistry. 31:9445-9450
When chicken liver pyruvate carboxylase was incubated with either H14CO3- or gamma-[32P]ATP, a labeled carboxyphospho-enzyme intermediate could be isolated. The complex was catalytically competent, as determined by its subsequent ability to transfer
Publikováno v:
Archives of Biochemistry and Biophysics. 284:98-105
When sheep liver pyruvate carboxylase was diluted below 4 EU/ml, it underwent inactivation involving two kinetically distinct processes, i.e., a rapid initial burst followed by a slower second phase. The catalytic activity of the diluted enzyme event
Publikováno v:
European Journal of Biochemistry. 112:265-272
Using negative staining and electron microscopic tilting techniques in conjunction with modelling experiments, the fine structure of chicken, sheep and rat pyruvate carboxylases has been studied. The overall configuration appears to be a tetrahedron-
Publikováno v:
European Journal of Biochemistry. 62:125-130
The 2′,3′-dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation and on the following criteria was considered to be an effective affinity label. The magnesium complex of this derivative (Mg-oATP2−) was shown to be a linear comp
Publikováno v:
European Journal of Biochemistry. 133:201-206
Negatively stained enzyme-avidin complexes, seen at different stages of the titration of the biotin-binding sites on avidin with chicken liver pyruvate carboxylase, have been studied using electron microscopy. Formation of linear, unbranched polymers
Autor:
Nelson F. B. Phillips, D. Bruce Keech, Mark A. Snoswell, Gregory J. Goodall, Paul V. Attwood, John C. Wallace
Publikováno v:
Annals of the New York Academy of Sciences. 447:169-188
Data from isotopic exchange studies and from experiments with 32P- and 14C-labeled enzyme-bound intermediates support the following description of the first partial reaction: (Formula: see text). From studies of the transfer of the carboxyl-group fro
Publikováno v:
Biochemistry. 21:3364-3370
In the presence of avidin, progress curves for the pyruvate carboxylase (EC 6.4.1.1) reaction show a marked decrease in the rate of reaction over a period of several minutes. These curves are quantitatively described by an equation in which the rate
Publikováno v:
Advances in Enzyme Regulation. 2:49-68
Summary The thesis has been advanced that the synthesis of P-enolpyruvate from pyruvate during gluconeogenesis in liver occurs by a two-reaction sequence involving the carboxylation of pyruvate to oxaloacetate and decarboxylation of this compound to
Autor:
D. Bruce Keech, John C. Wallace
Publikováno v:
Trends in Biochemical Sciences. 6:182-184
In this article we question how widespread the sigmoidal velocity response of an enzyme to a substrate or effector molecular is necessarily attributable to the homotropic co-operative binding of that ligand. By way of an example, we show how alternat