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pro vyhledávání: '"D. Botstein"'
Akademický článek
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Autor:
James E. Haber, Janos Demeter, Patrick O. Brown, M.P. Vaze, Audrey P. Gasch, Achille Pellicioli, Tim Stearns, Anna Malkova, Sang Eun Lee, D. Botstein, Marco Foiani
Publikováno v:
Cold Spring Harbor Symposia on Quantitative Biology. 65:303-314
Akademický článek
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Publikováno v:
Genetics. 132:337-350
We report the isolation and characterization of a synoptic set of site-directed mutations distributed throughout the single actin gene of Saccharomyces cerevisiae. Mutations were systematically targeted to the surface of the protein by identifying cl
Autor:
M. A. Hoyt, M. F. Manolson, D. Proteau, D Botstein, Robert A. Preston, Elizabeth W. Jones, J. Mulholland, A Stenbit, D Preuss, B. T. Roberts
Publikováno v:
Journal of Biological Chemistry. 267:14294-14303
Yeast vacuolar acidification-defective (vph) mutants were identified using the pH-sensitive fluorescence of 6-carboxyfluorescein diacetate (Preston, R. A., Murphy, R. F., and Jones, E. W. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 7027-7031). Vacuoles
Autor:
D, Botstein
Publikováno v:
Hospital Practice. 26:157-164
Yeast is an apparently strange "model" for the human genome, but it works because of the high degree of conservation in evolution between the primitive eukaryote and mammals. Moreover, the genic concentration in yeast--with almost no noncoding intron
Autor:
K M Hennessy, D Botstein
Publikováno v:
Cold Spring Harbor Symposia on Quantitative Biology. 56:279-284
One central event that must occur exactly once per cell division cycle is the replication of the genomic DNA. One defining observation of the eukaryotic cell cycle is that the nuclear DNA replicates during a short period (the S phase) and that during
Publikováno v:
The Journal of Cell Biology
Yeast mutants of cell cycle gene cdc48-1 arrest as large budded cells with microtubules spreading aberrantly throughout the cytoplasm from a single spindle plaque. The gene was cloned and disruption proved it to be essential. The CDC48 sequence encod
Publikováno v:
Proceedings of the National Academy of Sciences. 87:355-359
The 21-kDa GTP-binding Ypt1 protein (Ypt1p) is required for protein transport from the endoplasmic reticulum to the Golgi complex in yeast extracts. Ypt1 antibodies block transport; this inhibition is alleviated by competition with excess purified Yp
Autor:
D Botstein
Publikováno v:
Breast Cancer Research : BCR