Zobrazeno 1 - 7
of 7
pro vyhledávání: '"D W, Murhammer"'
Autor:
C, Mitchell-Logean, D W, Murhammer
Publikováno v:
Biotechnology and bioengineering. 56(4)
The effect of bcl-2 expression on cell viability and recombinant protein synthesis was investigated in the Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Fivetrade mark) insect cell lines. It was found that coinfection with a bacul
Publikováno v:
Biotechnology and bioengineering. 55(6)
Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Five) cell lines during growth and following viral infection in suspension cultures in order to develop a better under
Publikováno v:
Glycoconjugate journal. 16(12)
Glycosylation, the most extensive co- and post-translational modification of eukaryotic cells, can significantly affect biological activity and is particularly important for recombinant glycoproteins in human therapeutic applications. The baculovirus
Publikováno v:
Biotechnology and bioengineering. 70(5)
A new method for the preparation of the glycoforms of bovine alpha(1)-acid glycoprotein (AGP) is described relying on affinity-reversed micellar extraction and separation (ARMES). This method has proven effective in separating structurally similar gl
Autor:
M A, Saarinen, D W, Murhammer
Publikováno v:
In vitro cellulardevelopmental biology. Animal. 36(6)
The production of recombinant secreted alkaline phosphatase protein in virally infected insect cells was studied in shaker flask and high aspect rotating-wall vessel (HARV) culture. Two commonly used cell lines, Spodoptera frugiperda Sf-9 (Sf-9) and
Publikováno v:
Biotechnology and bioengineering. 63(5)
The Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell line has received considerable attention as a host for the baculovirus expression vector system. In the present study, suspension cultures were used to compare Tn-5B1-4 cell aggregates and cells
Publikováno v:
Biotechnology and bioengineering. 55(1)
The purpose of this study was to develop non-invasive techniques to monitor the composition of cell culture media in insect cell bioreactors. Such a monitor could be used in conjunction with a fed-batch feeding scheme to ensure that cells are maintai