Výsledky vyhledávání - "D V, Pyshnyĭ"

[A convenient approach to the synthesis of the phosphoramidite non-nucleotide monomers for the preparation of functionalized oligonucleotide derivatives]

Autor: M S, Kopriushkin, D V, Pyshnyĭ

Publikováno v: Bioorganicheskaia khimiia. 38(6)

A simple approach to the synthesis of phosphoramidite synthons for non-nucleotide inserts using 4-(2-(4,4'-dimethoxytrityloxy)ethyl)morpholine-2,3-dione as a key precursor is suggested. Using the method developed various inserts have been introduced

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1db5b835a59eede800d287922ec123a6
https://pubmed.ncbi.nlm.nih.gov/23547478

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[Oligonucleotide derivatives in the nucleic acid hybridization analysis. III. Synthesis and investigation of properties of oligonucleotides, bearing bifunctional non-nucleotide insert]

Autor: M S, Kupriushkin, D V, Pyshnyĭ

Publikováno v: Bioorganicheskaia khimiia. 38(6)

Non-nucleotide phosporamidites were synthetized, having branched backbone with different position of functional groups. Obtained phosphoramidite monomers contain intercalator moiety--6-chloro-2-methoxyacridine, and additional hydroxyl residue protect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ad02d29bbf40d6f0ea06b5129259e92e
https://pubmed.ncbi.nlm.nih.gov/23547474

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[Synthesis and properties of methylene carboxamide mimetics of nucleic acids based on morpholine nucleosides]

Autor: T V, Abramova, M F, Kasakin, Iu V, Tarasenko, A A, Lomzov, V V, Koval', D V, Pyshnyĭ, V N, Sil'nikov

Publikováno v: Bioorganicheskaia khimiia. 38(4)

Uracyl and adenine containing oligocarboxamide mimetics of nucleic acids based on morpholine nucleosides (MorGly) are synthesized using peptide chemistry methods. Conditions for an analysis of homogeneity of protonated at physiological pH oligomers u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1d00fa95ef6591bec02909b35d06f4ef
https://pubmed.ncbi.nlm.nih.gov/23189560

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[Considering the oligonucleotides secondary structures at thermodynamic and kinetic analysis of the DNA-duplexes formation]

Autor: A A, Lomzov, D V, Pyshnyĭ

Publikováno v: Biofizika. 57(1)

The influence of secondary structures of DNA oligonucleotides on thermodynamics and kinetics at the formation of their bimolecular complexes (duplexes) has been studied. The models considering inherent secondary structures of duplex components and th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ca0e1cb693e34679d10b90d64f033e65
https://pubmed.ncbi.nlm.nih.gov/22567908

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[Oligonucleotide derivatives in the nucleic acid hybridization analysis. II. Isothermal signal amplification in process of DNA analysis by minisequencing]

Autor: E V, Dmitrienko, E A, Khomiakova, Pyshnaia, A G, Bragin, V E, Vedernikov, D V, Pyshnyĭ

Publikováno v: Bioorganicheskaia khimiia. 36(6)

The isothermal amplification of reporter signal via limited probe extension (minisequencing) upon hybridization of nucleic acids has been studied. The intensity of reporter signal has been shown to increase due to enzymatic labeling of multiple probe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::472fb47d3ebd1ad2f476d62e686ecc9e
https://pubmed.ncbi.nlm.nih.gov/21317947

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[Oligonucleotide derivatives in the nucleic acid hybridization analysis. I. Covalent immobilization of oligonucleotide probes onto the nylon]

Autor: E V, Dmitrienko, I A, Pyshnaia, D V, Pyshnyĭ

Publikováno v: Bioorganicheskaia khimiia. 36(5)

The features of UV-induced immobilization of oligonucleotides on a nylon membranes and the effectiveness of enzymatic labeling of immobilized probes at heterophase detection of nucleic acids are studied. Short terminal oligothymidilate (up to 10 nt)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::73570ac55cafb5cab7ccc59e029ba3b2
https://pubmed.ncbi.nlm.nih.gov/21063457

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[Thermodynamic description of oligonucleotide self-association in DNA concatamer structures]

Autor: N S, Filippov, A A, Lomzov, D V, Pyshnyĭ

Publikováno v: Biofizika. 54(3)

A scheme of the formation of concatamer structures consisting of two different oligonucleotides has been considered. It was shown that, in the general case, the dependence of the concentration of oligonucleotide components on temperature cannot be fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e83daceff29937548223889d1dc8d24f
https://pubmed.ncbi.nlm.nih.gov/19569499

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[Enhancement of hybridization analysis efficacy by means of controlled fragmentation of analyzed DNA]

Autor: O A, Vinogradova, I A, Pyshnaia, V F, Zarytova, E M, Ivanova, D V, Pyshnyĭ

Publikováno v: Molekuliarnaia biologiia. 41(1)

The influence of fragmentation of analyzed DNA amplicon on the efficacy of specific sequence detection by means of heterophase hybridization analysis was investigated. The detection of DNA sequence was carried out colorimetrically after introduction

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fdfc2898db92d82aa7af3c07c7f8790f
https://pubmed.ncbi.nlm.nih.gov/17380903

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[The operation of tracheostomy: mistakes and complications]

Autor: S I, Kubyshkin, V A, Gorbunov, D V, Pyshnyĭ

Publikováno v: Voenno-meditsinskii zhurnal. 327(11)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1b27d416df18e6054aa52cc3d442dc3d
https://pubmed.ncbi.nlm.nih.gov/17300058

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[Determination of Herpes simplex virus DNA with the use of solid-phase methods for the detection of PCR products]

Autor: V A, Rar, D A, Terekhova, D V, Pyshnyĭ, I A, Pyshnaia, O V, Morozova

Publikováno v: Zhurnal mikrobiologii, epidemiologii i immunobiologii. (3)

To determine DNA of herpes simplex virus (HSV), types 1 and 2, the polymerase chain reaction (PCR) method was developed with the subsequent detection of amplification products by means of electrophoresis or the molecular hybridization of nucleic acid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d3279cdf6ff7ae206c4d27a888fc4b3a
https://pubmed.ncbi.nlm.nih.gov/16830598

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