Výsledky vyhledávání - "D S, Linthicum"

Antibody-based Fluorescence Polarization Assay to Screen Combinatorial Libraries for Sweet Taste Compounds

Autor: D. S. Linthicum, J. Patel, N. Cairns

Publikováno v: Combinatorial Chemistry & High Throughput Screening. 4:431-438

Technological advances in instrumentation, chemical synthesis methods, molecular biology and biochemistry have fueled the recent growth in high throughput screening. Assays are available in a vast range for formats, including fluorescence, luminescen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b99407f7dfb4fe19e2d5a96e0527a411
https://doi.org/10.2174/1386207013331002

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The three-dimensional structure of a complex of a murine Fab (NC10.14) with a potent sweetener (NC174): an illustration of structural diversity in antigen recognition by immunoglobulins

Autor: Paul A. Ramsland, Lin Shan, Allen B. Edmundson, D. S. Linthicum, Zhao-chang Fan, J. M. Anchin, Luke W. Guddat, C. Broomell

Publikováno v: Journal of Molecular Biology. 302:853-872

The three-dimensional structure of a complex of an Fab from a murine IgG2b(lambda) antibody (NC10.14) with a high potency sweet tasting hap- ten, N-(p-cyanophenyl)-N'-(diphenylmethyl)-N"-(carboxymethyl)guan idine (NC174), has been determined to 2.6 A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b197194b3a359b02182c9a54b7fed947
https://doi.org/10.1006/jmbi.2000.4083

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Ligand-induced domain movement in an antibody fab: molecular dynamics studies confirm the unique domain movement observed experimentally for fab NC6.8 upon complexation and reveal its segmental flexibility 1 1Edited by I. Wilson

Autor: Janos M. Varga, Bernd M. Rode, Klaus R. Liedl, D. S. Linthicum, Christoph A. Sotriffer

Publikováno v: Journal of Molecular Biology. 278:301-306

Two molecular dynamics simulations were carried out for the antibody Fab NC6.8, both with and without the guanidinium sweetener ligand NC174, in order to assess the segmental flexibility as well as the conformational changes upon ligand binding. Traj

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https://doi.org/10.1006/jmbi.1998.1684

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ABGEN: A Knowledge-Based Automated Approach for Antibody Structure Modeling

Autor: Chhabinath Mandal, Shankar Subramaniam, D. S. Linthicum, Jerry M. Anchin, B. D. Kingery

Publikováno v: Nature Biotechnology. 14:323-328

Immunoglobulin (Ig) amino acid sequences are highly conserved and often have sequence homology ranging from 70 to 95%. Antigen binding fragments (Fab), variable region fragments (Fv), and single chain Fv (scFv) of more than 50 myeloma proteins and mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4273261d2bad9220736ab274f9242bbe
https://doi.org/10.1038/nbt0396-323

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Intramolecular signaling upon complexation

Autor: D. S. Linthicum, Lin Shan, Zhao-chang Fan, R. Rosauer, Kim N. Andersen, Luke W. Guddat, Allen B. Edmundson

Publikováno v: The FASEB Journal. 9:101-106

Crystal habits can be used as indicators of conformational changes in their constituent proteins. As in the conversion of unliganded hemoglobin to the oxygenated form, the addition of a small hapten to a suspension of platy crystals of an unliganded

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee52924b4478480da2b9653a47d5aa92
https://doi.org/10.1096/fasebj.9.1.7821748

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Identification of residues in monoclonal antibody NC10.8 that bind to the sweetener N-(p-cyanophenyl)-N'-(diphenylmethyl)guanidinoacetic acid by using radioligand binding, absorption and fluorescence spectroscopy, computer-aided molecular modeling, and site-directed mutagenesis

Autor: J M Anchin, P R Droupadi, G E DuBois, M S Kellogg, S Nagarajan, J S Carter, D S Linthicum

Publikováno v: The Journal of Immunology. 153:3059-3069

The interactive residues for mouse mAb NC10.8, which binds a superpotent guanidinium sweetener N-(p-cyanophenyl)-N'-(diphenylmethyl)guanidinoacetic acid with high affinity (Kd = 5 nM), were examined by using radioligand competitive binding, photoaffi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ea0ab41a3f331e5a923160b1034d80f5
https://doi.org/10.4049/jimmunol.153.7.3059

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Spectroscopic evidence for charge-transfer complexation in monoclonal antibodies that bind opiates

Autor: E. A. Meyers, P. R. Droupadi, D. S. Linthicum

Publikováno v: Journal of Protein Chemistry. 13:297-306

Molecular complexes of four monoclonal anti-morphine antibodies (mAb) with the opiate ligands morphine, oxymorphone, and naloxone were studied using UV-VIS absorption spectroscopy. Although strong overlaps in the absorption spectra of the antibodies,

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https://doi.org/10.1007/bf01901562

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Histamine-Induced Micro vascular Leakage in Pial Venules: Differences Between the SJL/J and BALB/c Inbred Strains of Mice

Autor: D. S. Linthicum, E. L. Orr, C.J.R. Welsh, B. V. Sapatino, B. F. Bebo, T. Yong

Publikováno v: Journal of Neurotrauma. 11:161-171

The actions of histamine on pial venule leaky site formation were measured intravitally in two inbred strains of mice (BALB/c and SJL/J). Pial venules were visualized using a cranial window microscopy technique, and microvascular leaky site formation

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https://doi.org/10.1089/neu.1994.11.161

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Cloned mouse cerebrovascular endothelial cells that maintain their differentiation markers for factor VIII, low density lipoprotein, and angiotensin-converting enzyme

Autor: C.J.R. Welsh, C. A. Smith, B. V. Sapatino, D. S. Linthicum, B. F. Bebo

Publikováno v: In Vitro Cellular & Developmental Biology - Animal. 29:923-928

This communication describes a relatively novel cell culture technique for the isolation of cerebrovascular endothelial cells from three strains of inbred mice. Cerebrovascular endothelial cells were identified by their morphology, the presence of Fa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02305db51d8a4e7d56d0198f7f19a216
https://doi.org/10.1007/bf02634230

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