Zobrazeno 1 - 10
of 11
pro vyhledávání: '"D M, Zimmer"'
Publikováno v:
Environmental and molecular mutagenesis. 33(3)
We compared the lambda cII/cI transgenic mutation assay described by Jakubczak et al. [(1996): Proc Natl Acad Sci USA 93:9073-9078] to the previously established Big Blue assay. Genomic DNA isolated from liver, spleen, and lung tissue of control or e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::99a8f49fff6295f24c353dc89eb361e9
https://pubmed.ncbi.nlm.nih.gov/10334627
https://pubmed.ncbi.nlm.nih.gov/10334627
Publikováno v:
Environmental and molecular mutagenesis. 33(2)
We tested the ability of a series of known genotoxic agents to cause mutations at the hprt locus in peripheral blood T-lymphocytes of cynomolgus monkeys as measured by the ability to form clones in the presence of 6-thioguanine. Ethylmethane sulfonat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::211633d49cc77820baa7149946172c51
https://pubmed.ncbi.nlm.nih.gov/10217066
https://pubmed.ncbi.nlm.nih.gov/10217066
Publikováno v:
Environmental and molecular mutagenesis. 33(2)
Big Blue mice harbor a recoverable transgene in a lambda/LIZ shuttle vector. In the standard assay, in vivo mutations are measured in the bacterial lacI gene using a labor-intensive color plaque assay. Applying a simpler assay [Jakubczak et al. (1996
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c6c7cdb6cf430527c2f4b5e70a2c59fc
https://pubmed.ncbi.nlm.nih.gov/10217067
https://pubmed.ncbi.nlm.nih.gov/10217067
Publikováno v:
Environmental and molecular mutagenesis. 33(1)
We have been studying in vivo mutagenesis at the hypoxanthine phosphoribosyl transferase (hprt) locus in cynomolgus monkey T-lymphocytes. This primate model allows us to study mutations and their kinetics under well-controlled conditions. Previously,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ba42ac014d7bb36f12460752bc35af15
https://pubmed.ncbi.nlm.nih.gov/10037322
https://pubmed.ncbi.nlm.nih.gov/10037322
Publikováno v:
Environmental and molecular mutagenesis. 32(4)
We examined several experimental parameters of the lambda cI/cII transgenic mutation assay. In the assay, clear plaque lambda phage mutants are identified in a positive selection scheme following rescue of the lambda/LIZ shuttle vector from frozen ti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::679815f0edcf97a6738a0604729f4a70
https://pubmed.ncbi.nlm.nih.gov/9882006
https://pubmed.ncbi.nlm.nih.gov/9882006
Publikováno v:
Environmental and molecular mutagenesis. 32(3)
The optimization of the mouse lymphocyte Hprt mutation assay has been impeded by the relatively poor growth potential of mouse T-cells in vitro, which leads to low cloning efficiencies (CEs) and limited expansion of Hprt mutant clones for molecular a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e7e6f3a0ea73f46d31d2a0394412d0d5
https://pubmed.ncbi.nlm.nih.gov/9814438
https://pubmed.ncbi.nlm.nih.gov/9814438
Autor:
D M, Zimmer, C S, Aaron
Publikováno v:
Environmental and molecular mutagenesis. 29(2)
We have monitored mutant frequency at the HPRT locus in peripheral blood lymphocytes of cynomolgus monkeys using a clonal assay in which mutants are selected by resistance to 6-thioguanine. Among untreated animals, the mean spontaneous mutant frequen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::75970e2505531253091a2f7f813c0a2b
https://pubmed.ncbi.nlm.nih.gov/9118963
https://pubmed.ncbi.nlm.nih.gov/9118963
Publikováno v:
Environmental and molecular mutagenesis. 28(4)
Big Blue Rat-2 cells were evaluated for mutagenesis and mutational spectra (spontaneous and ethylnitrosourea [ENU]-induced). Survival, mutant frequency, population doubling time, and kinetics of mutant increase (to 120 hr) were determined. Exposures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7e3e09e4de4fd08e4e76a98a1c4c80de
https://pubmed.ncbi.nlm.nih.gov/8991060
https://pubmed.ncbi.nlm.nih.gov/8991060
Publikováno v:
Environmental and molecular mutagenesis. 18(3)
We have investigated the use of cynomolgus monkeys (Macaca fascicularis) as a model of somatic cell mutagenesis in non-human primates. Using techniques described by Albertini (Mutation Research 150:411-422, 1985) for similar studies in humans, the fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b33532d8d09c0a951cd6543dafcaf5d
https://pubmed.ncbi.nlm.nih.gov/1915311
https://pubmed.ncbi.nlm.nih.gov/1915311
Publikováno v:
Cancer research. 48(1)
CC-1065 is a very potent antitumor antibiotic which binds in the minor groove of DNA with alkylation at N-3 of adenine. Since CC-1065 caused delayed deaths in mice at therapeutic doses, analogues were prepared whose antitumor and biochemical activiti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::730604a34daf88e92a210edfd66e7730
https://pubmed.ncbi.nlm.nih.gov/3334998
https://pubmed.ncbi.nlm.nih.gov/3334998